Study On The Intervention Mechanism Of Jinshui Liujun Decoction Based On The Role Of Macrophage M1/M2 Phenotype Polarization In COPD Mouse Mode

Objective: To investigate the effect of macrophage M1/M2 phenotype differentiation on the development of COPD patients and the mechanism of Jinshui Liujun decoction in the treatment of COPD.Methods: Thirty-two ICR mice were randomly divided into 4 groups,namely blank group,model group,western medicine group and Chinese medicine group,with 8 mice in each group.A mouse model of COPD was prepared by simple smoking for 12 weeks.And from the5 th week,corresponding drugs were given by intragastric administration.The general condition of mice was observed,and the differences in hair,spirit and body weight of mice in each group were assessed.Lung tissues of mice were sacrificed for HE and ELISA tests to evaluate inflammation,lung function to evaluate COPD severity,M2 macrophage-related markers,and M1/M2 polarization of macrophages were evaluated by flow cytometry and immunofluorescence tests.Results: Compared with blank group,the levels of MV,PIF and PEF in model group were decreased(P<0.05).Compared with model group,the levels of MV,PIF and PEF in all experimental groups were increased(P<0.05),among which the TCM group was the most significant.Compared with HE staining,the lung tissue of mice in blank group was intact,and there was no obvious rupture and fusion of alveoli.Alveolar rupture and fusion were observed in the model group,which was an obvious pulmonary tissue sign of COPD.Compared with the model group,alveolar rupture and fusion were significantly reduced in the Chinese medicine group and the western medicine group.There was no significant difference in alveolar destruction and inflammation between the Chinese medicine group and the Western medicine group.Compared with the result of ELSIA,IL-6 and IL-8 in the model group were significantly increased,indicating obvious inflammation.Compared with the result of ELSIA,IL-6 and IL-8in the model group were significantly increased,indicating obvious inflammation.Compared with model group,IL-6 and IL-8 decreased significantly in western medicine group and Chinese medicine group.The polarization direction of macrophages was compared,and the results of immunofluorescence experiment showed that CD68 and CD206 were positively expressed in different degrees in all groups.The expressions of CD68 and CD206 were not obvious in the blank group,indicating that macrophages were not activated.Compared with other groups,CD68 positive expression was the most obvious in the model group,suggesting that macrophages were activated in the lung tissue of the model group.CD206 positive expression was obvious in the Chinese medicine group and the western medicine group,suggesting that macrophages in lung tissue had a tendency of M2 polarization after treatment.The expressions of M1 and M2 macrophages were detected by flow cytometry.CD86 positive proportion represents the M1 polarization proportion of macrophages,CD163 positive proportion represents the M2 polarization proportion of macrophages,and CD68+CD206 positive proportion represents the total macrophages proportion in lung tissue.The results showed that compared with the other three groups,the proportion of macrophages labeled by CD68+CD206 was the highest in the model group,and the lowest in the blank group.Compared with model group,the M2/M1 ratio in lung tissue of TCM group and western medicine group was significantly increased.Conclusion: Jinshui Liujun decoction may be effective in the treatment of COPD by inhibiting the M1 phenotype differentiation of macrophages and promoting the M2 phenotype differentiation,and inhibiting the occurrence of inflammatory response.