Effects Of Shennao Fuyuan Decoction On The Expression Of PPARγ, ENOS And Angiogenesis In Rats With Cerebral Ischemia-reperfusion Mode

Objective: To investigate the effects of Shennao Fuyuan Decoction on peroxisome proliferators-activated receptor γ(PPARγ)and endothelial nitric oxide synthase(e NOS)expression and cerebral angiogenesis in rats with cerebral ischemia-reperfusion injury.Methods: SD rats are randomly divided into sham-operated group and model preparation group.Model preparation group were used to establish the middle cerebral artery occlusion(MCAO)model,and then divided into model group,Shen nao Fuyuan Decoction group(Traditional Chinese Medicine Group,TCM)and Butylphthalide group(Western Medicine Group)by random number table method.Each component was divided into three phases of 3d,7d and14 d.Sham-operated group and model group were given equal volume of distilled water and equal volume of normal saline by intraperitoneal injection;TCM group was given equal volume of Shennao Fuyuan Decoction and equal volume of normal saline by intraperitoneal injection;Western medicine group was given equal volume of distilled water and equal volume of Butylphthalide mixed suspension by intraperitoneal injection.The extent of neurologic deficit in rats was assessed with m-NSS scores;HE staining was used to observe the morphological changes of brain cells.TTC staining was used to detect the volume of cerebral infarction;the immunofluorescence was used to observing neovascular endothelial cell proliferation;RT-PCR were used to detect the relative of PPARγ and e NOS m RNA expression in rat brain tissue.Results: 1.Neurological function score: there was no neurological deficit in the sham-operated group.Compared with the model group,the symptoms of neurological deficits were significantly improved in both the TCM group and the western medicine group(P<0.05),and there was no significant difference between the TCM group and the western medicine group(P>0.05).2.Cerebral infarction volume: The whole brain tissue of the sham-operated group was all red,and no obvious areas of cerebral infarction were seen.Compared with the sham-operated group,the model preparation group can see obvious white infarction areas.The volume of cerebral infarction of model group didn’t change significantly,yet the TCM group and the western medicine group significantly reduced infarct volume 3d to 7d,compared with model group at the same time were significant(P<0.05).3.HE staining: The arranges of the neurons of the cerebral tissue of the sham-operated group were in an orderly manner,with normal circular and oval shapes.The nuclear structure is obvious and the nuclear kernels are clear.The gaps around the brain tissue cells in the model group increased,most of the cortex region was deeply stained and showed the nuclear contraction of the cells,the change of empty foam samples,and blurred structures.Neuroplastin ischemic changes in the TCM group and western medicine group were alleviated,and normal cells had increased significantly compared with the model group.Only a small amount of cell edema,deep dye,and nuclear contraction change can be seen.4.Immune fluorescence: There were almost no common positioning of CD31 and KI67 in the sham-operated group,and there were fewer angiogenesis.Compared with the sham-operated group,the common positioning of CD31 and KI67 around the model group’s brain tissue infarction area was increased obviously.Compared with the model group,the common positioning of CD31 and KI67 of the TCM group and the western medicine group increased significantly,suggesting that the number of neovascular endothelial cells increased significantly.5.RT-PCR: There was a small amount of PPARγ and e NOS m RNA expression in the sham-operated group.Compared with the sham-operated group,the relative expression of PPARγ and e NOS m RNA in each model preparation group has increased(P<0.05);In the same phase,compared with model group,the relative expression volume of PPARγ and e NOS m RNA in the TCM group and western medicine group increased,There was no difference in PPARγ and e NOS m RNA expression between the TCM group and western medicine group(P>0.05).Conclusion: Shennao Fuyuan Decoction can effectively improve the neurological defects after cerebral ischemia reperfusion in rats,promote angiogenesis and neurological function recovery,and the mechanism may involve the activation of PPARγ/e NOS signaling pathway.