Study On The Effect Of Antler Glue On Improving Erectile Function And Its Mechanism Of Action In Diabetic ED Rat

Objectives: To study the effects of deer antler gum on hair color,performance symptoms,blood glucose,body mass and pe nile testicular mass and blood glucose of DMED rats by establishing a diabetic mellitus erectile dysfunction(DMED)SD rat model;the effects on erectile function of DMED rats;the effects on serum T,LH and FSH of DMED rats;the effects on penile corpus c avernosum tissue morphology of DMED rats and e NOS and c GMP,VEGF protein and m RNA expression in DMED rats.Methods: Seventy SD male rats were adaptively fed for 1 week and divided into blank and modeling groups.Ten rats in the blank group were fed with no rmal diet,and 60 rats in the modeling group were fed with high-sugar and high-fat diet for 4 weeks,and then type 2 diabetes mellitus(T2DM)model rats were prepared by intraperitoneal injection of 35 mg/kg streptozotocin(STZ).after successful modeling of T2 DM,the rats were continued to be fed with high-sugar and high-fat diet for 8 weeks,and the DMED rat model was screened with apomorphine.The surviving 35 DMED rats were randomly divided into model group,tadalafil group,antler gum group,antler gum +metformin group and metformin group,7 rats in each group.The normal group and model group were gavaged with purified water,and the remaining groups were gavaged with tadalafil 0.2 mg/kg/d,antler gum 1.2 g/kg/d and metformin 200 mg/kg/d corresponding drugs for28 days.After 28 days of administration,the levels of serum testosterone(T),luteinizing hormone(LH),follicle-stimulating hormone(FSH)and cyclic guanosine monophosphate(c GMP)in penile cavernosa of rats were measured by ELISA,and the ex pression of nitric oxide synthase(e NOS)and vascular endothelial growth factor(VEGF)-related proteins in penile cavernosa of rats were detected by Western blot.The m RNA levels of e NOS,c GMP and VEGF were detected by RT-q PCR.And HE staining technique wa s applied to observe the repairing effect of deer antler gum on the penile cavernous tissue of DMED rats.The experimental data were analyzed by SPSS 25.0 statistical software.Results: After the drug intervention,the general performance such as hair color and response sensitivity were improved in deer antler gum group,metformin group and deer antler gum + metformin group compared with the other two groups of DMED rats.There was no statistically significant difference in body mass between the DMED model rats and the blank rats(P<0.05);however,there was no significant difference in body mass between the DMED groups(P>0.05);the metformin group and the antler gum + metformin group were relatively heavier than the model group.Metformin group and deer antler gum + metformin group had relatively lower blood glucose relative to the model group,tadalafil group and deer antler gum group(P<0.05),and there was no statistically significant difference in blood glucose between the metformin group and dee r antler gum +metformin group compared to each other.After induction by APO,the number of erections in all groups was less than that in the blank group,(P<0.05)among which,the number of erections in the model group rats were induced by APO;after 8 w eeks of drug intervention,the number of erections in the tadalafil group,metformin group,deer antler gum group and metformin + deer antler gum group rats were different compared with the model group rats(P<0.05)and the deer antler gum + metformin group compared with the tadalafil group was relatively The number of erections was better in the deer antler gum+ metformin group compared with the tadalafil group,but there was no statistical difference.The serum T levels of DMED rats in all groups were significantly lower(P<0.05);compared with the model group,the serum T levels of deer antler gum group,deer antler gum+ metformin group,metformin group and tadalafil group were higher,and the differences were statistically different(P < 0.05);the serum T levels of deer antler gum + metformin group were higher than those of deer antler gum group and metformin group,and there was no significant difference compared with the tadalafil group(P >0.05)LH and FSH levels were not significantly d ifferent in each group(P>0.05).Compared with the blank group,the expression levels of e NOS,c GMP,VEGF protein and m RNA in the penile corpus cavernosum tissues of DMED rats were significantly lower in all groups(P<0.05);compared with the model group,the expression levels of e NOS,c GMP,VEGF protein and m RNA in the penile corpus cavernosum of deer antler gum group,deer antler gum + metformin group and tadalafil group were increased,and the differences were statistically The expression levels of e NOS,c GMP,VEGF pr otein and m RNA were higher in the deer antler gum + metformin group than in the deer antler gum group,and there was no significant difference compared with the tadalafil group(P>0.05).The penile cavernous tissue was repaired to different degrees in the deer antler gum group,deer antler gum + metformin group,tadalafil group and metformin group,and the number of erythrocytes in the penile cavernous vessels was increased compared with the model group,but the difference in connective tissue was not signi ficant,and the pathological changes were between the blank group and the model group.Conclusion: Deer antler gum improved the hair color and performance symptoms of DMED model rats,but could not increase the body weight of rats,could increase the penil e mass,and had no significant effect on the testicular mass of left and right sides.Deer antler gum can improve the erectile function of DMED rats,and combined with hypoglycemic drugs,can play a better therapeutic effect.Deer antler gum could increase the serum T level in rats,but had no significant effect on LH and FSH level,which might be because deer antler gum could improve the microenvironment of T production and thus increase the serum T level,rather than directly regulating the HPTA axis to i ncreas e the T level.Deer antler gum could activate NO/c GMP pathway in DMED rats and promote e NOS and c GMP protein expression in penile corpus cavernosum tissue.Deer antler gum could reduce the degree of penile tissue damage and promote the expression of VEGF protein in DMED rats.