Study On The Characteristics Of Lymph Node Metastasis Of Papillary Thyroid Carcinoma And The Molecular Mechanism Of MiR-151-5p Promoting Its Metastasi

Correlation analysis of papillary thyroid carcinoma lymph node metastasis with clinical pathology and preoperative hematological indexesObjective Thyroid carcinoma is the most common malignant tumor in the endocrine system.papillary thyroid carcinoma(PTC)accounts for about 80%of all thyroid cancer types.Surgery is the first choice of treatment for PTC,and postoperative radionuclide therapy and TSH suppression therapy can improve the prognosis of some patients.However,studies have reported that 40%～60%of PTC patients in the first patient has occurred lymph node metastasis that associated with prognosis and is one of the main factors influencing the prognosis of PTC.Determine of prognostic factors optimize treatment selection of thyroid papillary carcinoma,reduce complications and unnecessary treatment to improve the survival rate of patients.Analysis of correlation between clinical features and central lymph node metastasis helps to more accurately determine the lymph node status for central lymph node dissection and provide a theoretical basis.It has important significance on the prognosis of PTC.Methods A retrospective analysis was made to 200 cases of thyroid lobectomy+ipsilateral central lymph node dissection in patients with unilateral PTC resection.The correlation between clinical pathological features and preoperative blood indexes was analyzed statistically.Results1.Univariate analysis showed that PTC patiens with age<45 years,tumor size>1cm,capsule invasion increased significantly central lymph node metastasis rate.There is a correlation between preoperative hematological index(RBC level≥4.405×1012/L,Tg level≥21.15ng/ml,total protein level≥78.25g/L,CA125 level≥5.6U/ml,globulin level≥29.05g/L,CA153 level≥6.75U/ml)and high lymph node transfer rate.2.Multivariate analysis showed that capsular invasion,high globulin level(≥29.05g/L),high CA153 level(≥6.75U/ml)is risk factors to predict central cervical lymph node metastasis in PTC patients.Conclusion Patients with age<45 years,tumor size>1cm,capsular invasion and preoperative blood indexes(globulin level≥29.05g/L,CA153 level≥6.75U/ml)correlated with PTC lymph node metastasis.For these patients,prophylactic central lymph node dissection should be considered.The molecular mechanism of mir-151-5p in regulating Papillary thyroid carcinoma metastasisObject papillary thyroid carcinoma(PTC)is the most common malignant tumor of the thyroid,which is easy to occur cervical lymph node metastasis.However,the mechanism of PTC metastasis is still unclear.MiR-151-5p is a microRNA(miRNA)mature body,through pairing with the target gene mRNA guided silencing complex(RISC)to degrade mRNA or hinder mRNA translation,which negatively regulate gene expression at the post-transcriptional level.In recent years,more and more studies on miRNA have been found because of the association between miRNA and occurrence and development in many diseases,especially tumors.Previous studies have shown that abnormal expression of miR-151-5p in PTC is closely related to the biological behavior of tumor invasion and metastasis.However,the function of miR-151-5p in PTC lymph node metastasis has not been reported.The possible mechanism of miR-151-5p has become urgent problems to be solved in promoting PTC lymph node metastasis.Methods1.The expression levels of miR-151-5p and miR-151-3p were detected by RT-PCR in PTC tissues and matched para cancerous tissues from 200 patients with PTC.Clinical datas were collected to analyze the relationship between miR-151-5p and clinical pathological features.2.RT-PCR technology was used to detect the expression level of RhoGDIa mRNA in the above tissues.Then we analyzed the relationship between RhoGDIa mRNA and PTC clinical pathological features,miR-151-5p.3.Using Western blot to detect the expression of RhoGDIa protein in PTC tissues.Analyze the relationship between the expression of RhoGDIa protein and miR-151-5p.Results1.RT-PCR showed that the expression level of miR-151-5p was significantly higher than that of adjacent normal tissues(p=0.000)in PTC,the expression level of PTC in the metastasis group was significantly higher than that in non metastasis group(p=0.034);the expression of miR-151-3p was significantly higher than that of adjacent normal tissues(p=0.000)in PTC,but no significant difference in metastasis group and non metastasis group(p=0.658).76.5%of PTC showed low RhoGDIa mRNA level.2.Relationship between relative miR-151-5p expression and PTC clinicopathological features:abnormal expression of miR-151-5p is associated with gender,focality,invasion,lymph node metastasis and TNM stage at the first diagnosis(p=0.000,p=0.004,p=0.018,p=0.000 and p=0.000,respectively).Cases of high miR-151-5p expression level in metastatic group were significantly more than that in non metastasis group(111 vs 42,p=0.000).3.Relationship between RhoGDIa mRNA level and PTC clinicopathological features:abnormal expression of RhoGDIa mRNA is associated with gender,focality,invasion,lymph node metastasis and TNM stage(p=0.000,p=0.004,p=0.002,p=0.000 and p=0.000,respectively).Cases of low RhoGDIa expression level in metastatic group were significantly more than that in non metastasis group(111 vs 42,p=0.000).4.RT-PCR and Western blot showed that,compared with non metastasis group,miR-151-5p level was significantly increased;RhoGDIa protein was significantly down regulated in the metastatic group.The expression of miR-151-5p was negatively correlated with RhoGDIa.Conclusion The expression of miR-151-5p and RhoGDIa is related to the metastasis of PTC.While the expression of miR-151-5p is negatively correlated with the expression of RhoGDIa,suggesting that miR-151-5p may negatively regulate the expression of RhoGDIa gene to promote tumor metastasis.