Mining Of Candidate Genes In Response To Zinc Deficiency In Rice And Functional Study Of OsZIP1

Zinc is one of the essential trace elements for plants,animals and human body.Zinc deficiency will affect the yield and quality of rice,and excessive zinc will also affect the growth of plants,and eventually lead to plant death.Therefore,it is of great significance to mine and screen new zinc homeostasis regulatory genes for understanding the mechanism of zinc homeostasis regulation in rice and improving the accumulation of zinc elements in crops.In this study,17 different materials were used for transcriptome analysis and weighted gene co-expression network construction under zinc deficiency conditions.Under the condition of zinc deficiency stress,4976 difference-expressed genes were identified in zinc deficiency sensitive materials(KALIBOR0121,Shanyou836 and Sambha)and zinc deficiency tolerant materials(Luhuang Zhan,HASSAN and Fedearroz50).In GO enrichment analysis,biological processes such as REDOX processes,transcriptional response regulation and transmembrane transport,molecular functions such as REDOX enzyme activities,binding of iron and copper ions,and cellular components such as plastids,membranes,thylakoids and light systems were significantly enriched.In KEGG pathway analysis,phenylpropane biosynthesis pathway,glutathione metabolism,photosynthesis-antenna protein and various plant secondary metabolites biosynthesis pathway were significantly enriched.The enriched genes included transporters(YSL family,NRAMP family,HMP family and ABC family),transcription factors(MYB,NAC,WRKY,bHLH and ERF),protein kinase(WAK)and ROS homeostasis related genes(oxidase,laccase and cytochromic P450 gene).A total of 10 modules were identified by weighted gene coexpression network analysis(WGCNA),in which module E was significantly correlated with root length phenotype.Network analysis was performed on modules A,B,C and E to screen out some genes that might be related to zinc homeostasisWe also investigated zinc homeostasis related ZIP transporter family member OsZIP10,whose expression is induced by zinc deficiency or cadmium addition.In the experiments of zinc deficiency in vegetative growth stage,the knockout of OsZIP10 decreased the tolerance of rice to zinc deficiency,and the overexpression of OsZIP10 increased the tolerance of rice to zinc deficiency,suggesting that OsZIP10 had a positive regulatory effect on zinc deficiency tolerance of rice.At the maturity stage,zinc content was increased and cadmium content was decreased when OsZIP10 was overexpressed.