Effect Of Gegenqinlian Decoction And Its Drug Compatibility On Nonalcoholic Fatty Liver And Its Mechanism

Objective1.Use network pharmacology to speculate the potential approach of Gegenqinlian decoction in the treatment of non-alcoholic fatty liver disease2.To study the effects of Gegen Qinlian decoction(GGQLD)on metabolism-related factors: AMPK/SIRT1/AKT,in a Sprague-Dawley(SD)rat model of high-fat diet(HFD)induced non-alcoholic fatty liver(NAFL).Methods1.Network pharmacological techniques were used to analyze the potential mechanism of Gegenqinlian decoction in the treatment of non-alcoholic fatty liver disease.2.On the basis of the preliminary research results of the research group,combined with the prediction results of network pharmacology,SD rats with high fat diet were used to establish a non-alcoholic fatty liver disease model.Using the drug withdrawal method,the SD rats were randomly divided into: simvastatin group,gegenqinlian decoction group,pueraria lobata scutellaria baicalensis compatibility group,pueraria radix scutellaria baicalensis glycyrrhiza compatibility group,pueraria scutellaria baicalensis licorice compatibility group,scutellaria baicalensis licorice compatibility group,Model group and normal group,8 in each group.After 8 weeks of modeling,intragastric administration was given for 4 weeks.Collect materials at the end of the 12 th week and collect samples for indicator testing.3.The changes of TG,TC,AST,ALT,HDL-C and LDL-C were assayed by ELISA.Liver tissue morphology were examined by performing HE staining;TNF-α and IL-6 levels were detected by enzyme linked adsorption method;Western Blotting was used to detect the protein expressions of AMPK,AKT,SIRT1,SREBP-1c and FAS.Results1.Gegen Qinlian Decoction has 111 potential targets for the treatment of non-alcoholic fatty liver,involving biological processes such as aging,drug response,negative regulation of apoptosis process,positive regulation of DNA template transcription and other biological processes,as well as cancer pathways,hepatitis B,and TNF signaling pathways,HIF-1 signal pathway and other pathways.There are 36 core genes,including pathway-related genes SREBP-1c and SIRT1 that the research group studied in the early stage.2.The drug compatibility of Gegenqinlian decoction can reduce the degree of fat vacuolation,ballooning and cytoplasmoporotic degeneration in rat liver tissue induced by high fat diet.Simvastatin combined with Gegenqinlian decoction can improve the serum TC and TG levels of NAFL model rats(P<0.05).The level of serum LDL-C in Radix Scutellariae Radix,Radix Scutellariae Radix Glycyrrhiza Radix,Radix Scutellariae Radix Glycyrrhiza Radix could be decreased(P<0.05).Group of rhizoma coptidis,radix puerariae radix scutellariae compatibility with puerariae and scutellariae and coptidis decoction group compatibility of rhizoma coptidis,radix scutellariae,radix glycyrrhizae AST,ALT levels compared with model group,were significantly decreased(P < 0.05),radix puerariae radix scutellariae radix glycyrrhizae group compared with model group serum ALT levels decreased significantly(P < 0.05).3.Western blot results showed that high fat diet could inhibit the protein expressions of AMPK,SIRT1 and AKT in the liver tissues of SD rats(P<0.05),and simvastatin could improve the protein expressions of AMPK and AKT in the liver tissues of SD rats.Drug compatibility with puerariae and scutellariae and coptidis decoction can improve SIRT1 and AKT protein expression(P < 0.05),and there was no significant difference between groups(P > 0.05),group and radix puerariae radix scutellariae coptis with puerariae and scutellariae and coptidis decoction can increase the liver tissue of AMPK in protein expression.After high fat diet induction,the protein expressions of SREBP-1c and Fas in liver cells of model group were significantly increased(P<0.05).Compared with the model group,the protein expression levels of SREBP-1c and Fas in liver tissue of each drug group showed a decreasing trend(P<0.05).Conclusions1.Gegen Qinlian Decoction may treat NAFLD through related mechanisms such as insulin resistance,inflammatory factors,oxidative stress,cell apoptosis,and liver lipid synthesis.2.The drug compatibility of Gegen Qinlian Decoction reduces liver lipid deposition through AMPK/SIRT1/AKT signaling pathway,and improves liver steatosis in NAFL rats induced by high-fat diet.3.In the compatibility of Gegen Qinlian decoction,Gegen Qinlian Decoction group and Gegen Huangqin Huanglian group can improve the protein expression of AMPK in liver tissue...