| Objective(s): spinal cord injury(SCI)is a serious neurological injury common in clinical neurosurgery and orthopedics.Although great progress has been made in understanding the pathogenesis of SCI in recent years,there is currently no effective treatment for SCI.St em cells have the characteristics of proliferation and the ability of multidirectional differentiation,and can differentiate into nerve cells under some specific treatments.In this process,a variety of cytokines will be produced to stimulate the injured site and promote repair or regeneration.In addition,they also have anti-apoptosis,anti-inflammatory and other properties,so stem cells have broad application prospects in repairing spinal cord injury.Biological materials have good compatibility with c ells and various tissues.It has an important position in the field of nerve repair.The purpose of this study was to investigate the changes of the hind limb movement of rats after umbilical cord mesenchymal stem cells and neuroepithelial stem cells combine d with biomaterials were transplanted into rats with total transverse spinal cord injury,and to observe the survival of the transplanted stem cells,so as to provide certain theoretical and experimental basis for the treatment of spinal cord injury by bio materials combined with stem cell transplantation.Methods: In this study,the biocompatibility of stem cells was detected by CCK8.In order to verify the effect of stem cells combined with biomaterials on spinal cord injury,adult SD rats were used to make spinal cord transection model.Materials and stem cells we re transplanted along the injury site.SCI rats were implanted with labeled fluorescent stem cells and combined with the survival time of the materials.Behavioral test was performed within 3 months: BBB score and inclined plate test were used to evaluate the hindlimb movement of rats.Three months later,immunofluorescence staining was used to detect the expression of NF,NEUN,iba1 and GFAP in spinal cord injury rats without material and stem cell treatment.At the same time,hematoxylin-eosin staining(H ematoxylinEosin Staining,HE)and Nissl staining were used to observe the morphological changes of spinal cord cells in SCI rats treated with materials and stem cells.In addition,we also verified the effect of stem cell binding materials on spinal bladder function.Urine volume was measured and hematoxylin-eosin staining(Hematoxylin-Eosin Staining,HE)was used to observe the morphological changes of bladder epithelial cells and muscle layer and the size of bladder in SCI rats treated with materials and ste m cells.Further confirm the role of stem cells combined with biomaterials in SCI.Results: Our study found that biomaterials did not affect the vitality of cells,had no toxicity,and had good histocompatibility.At the same time,we also found that biom aterials combined with stem cells survived in SCI rats for one month.We also found that the movement of hind limbs of SCI rats was significantly improved after being treated with stem cells and biomaterials.In addition,the number of NF increased signifi cantly,the production of GFAP decreased significantly,and the number of microglia decreased significantly in SCI treatment group,indicating that stem cells and biomaterials can improve the hindlimb function and alleviate the pathological process of SCI in SCI rats.At the same time,the bladder function of SCI rats treated with stem cell combination materials was studied.It was found that the bladder of the treatment group was smaller than that of the untreated group,and the intravesical urine volume o f the treatment group was significantly less than that of the untreated group.HE staining of bladder sections showed that 90 days after SCI,compared with normal rats,the urinary tract epithelium was significantly thickened and the bladder detrusor bundl e fibers were obviously disordered in the control group and simple material group.In contrast,rats that received biomaterial-loaded stem cells showed a decrease in bladder wall thickness and bladder cross-sectional area.Transplantation of biomaterial-loaded stem cells can significantly restore motor and bladder function in SCI rats after injury.Conclusions: In the animal model of spinal cord injury,biomaterials and stem cells can improve the movement of hind limbs and improve the function of bladder i n rats with spinal cord injury,suggesting that biomaterials and stem cells may play an important role in the treatment of spinal cord injury.In addition,we further found that stem cell and material therapy can promote the growth of neurofilaments in spi nal cord injury,reduce the production of astrocytes and reduce the number of microglia.Our results show that the study of material and stem cells in the treatment of spinal cord injury is a promising strategy and is of great value in the treatment of spinal cord injury. |
PDF Full Text Request