The Correlation Between Effect Of Neoadjuvant Radiotherapy And Chemotherapy And IL-17A Expression In Rectal Cancer Tissues And Clinicopathologic

Background:Colorectal cancer is one of the most common malignant tumors in the world,and it is also the second leading cause of cancer-related deaths,with more than 1 million cases and about 500000 deaths each year.Neoadjuvant radiotherapy and chemotherapy refers to a treatment taken before the implementation of local treatment(surgery).At present,neoadjuvant radiotherapy and chemotherapy combined with surgery has become the recommended treatment for locally advanced rectal cancer(TNM Ⅱ,Ⅲ).Neoadjuvant therapy can better regulate the local growth of tumor,reduce the toxicity of treatment,increase the probability of sphincter retention,and then protect the quality of life of patients and improve functional outcomes.Some studies have shown that the expression of Th17 is positively correlated with the severity of cancer,and Th17 is gradually recruited into the tumor microenvironment in the process of tumor development.Based on the role of Th17 in rectal cancer,inhibiting the expression of Th17 in rectal cancer may be an effective strategy for the treatment of rectal cancer.IL-17 A is the first IL-17 family cytokine encoded by 150 AA.Most studies on the role of cytokines secreted by Th17 in colorectal diseases are focused on IL-17 A,At present,there is no report on the effect of neoadjuvant radiotherapy and chemotherapy on the expression of IL-17 A in rectal cancer,in order to explore the effect of neoadjuvant radiotherapy and chemotherapy on the expression of IL-17 A in rectal cancer.The expression levels of IL17 A in rectal cancer tissues and normal colorectal tissues were compared and analyzed.Objective :To compare the expression level of IL-17 A in rectal cancer tissue and normal intestinal tissue,and to explore the effect of preoperative neoadjuvant radiotherapy and chemotherapy on the expression of IL-17 A in tumor tissue and the correlation of clinicopathology,so as to provide basis for further research and clinic.Methods:The clinical data of rectal cancer patients who underwent surgery in our hospital from January 1,2021 to December 31,2022 were collected.According to whether they had received neoadjuvant radiotherapy and chemotherapy before operation,all patients were divided into two groups: group A(n = 60)and group B(n = 60).The expression of IL-17 A in tumor tissue and corresponding normal intestinal tissue of patients in group An and B was detected by immunohistochemical method,and compared with the relevant clinicopathological features(age,gender,tumor size,pathological stage,degree of differentiation,local lymph node metastasis,nerve infiltration,vascular invasion,etc.)Results:A total of 120 patients with rectal cancer in group A and B were included in this study.In group A,the positive expression of IL-17 A in rectal cancer was 20 cases(33.3%),and the negative expression was 40 cases(66.7%).The positive expression of IL-17 A in normal intestinal tissue was 12 cases(20.0%),and the negative expression was 48 cases(40.0%).In group B,the positive expression of IL-17 A in rectal cancer was 39 cases(65.0%),and the negative expression was 21 cases(35.0%).In normal intestinal tissue,the positive expression of IL-17 A was 25 cases(41.7%),and the negative expression was 35 cases(58.3%).The difference was statistically significant(P < 0.05).When IL-17 A was highly expressed in tumor tissues,the later the clinicopathological stage,the poorer the tumor differentiation,and the more prone to local lymph node metastasis,but there was no significant difference with gender,age,maximum diameter of tumor,vascular invasion,nerve infiltration and distant metastasis.The positive expression of IL-17 A in rectal cancer tissue in group A was20 cases(33.3%),and that in group B was 39 cases(65.0%).The positive rate in group A was significantly lower than that in group B(P < 0.05).Conclusions:1.The expression of IL-17 A in rectal cancer was higher than that in distal normal colorectal tissue.2.The higher the expression of IL-17 A,the later the tumor stage,the lower the degree of differentiation,and the more common local lymph node metastasis.3.Neoadjuvant radiotherapy and chemotherapy decreased the expression level of IL-17 A in rectal cancer and normal colorectal tissue.