Study On OCT/PAM Dual-modality Enhanced Imaging In Ophthalmology

With the improvement of life,people pay more and more attention to eye health.Visual impairment and even blindness caused by ophthalmic diseases seriously affect the quality of life of patients.Many early and recurrent eye diseases first appear in the retina and choroid,such as glaucoma,diabetic retinopathy,retinal vein occlusion and other eye diseases,which seriously affect their health.It is important to detect these diseases in the retina and choroid fundus.Therefore,the study of the morphology,physiology and pathology of the retina and choroid in the fundus of the eye is of great practical significance to explore various ophthalmic diseases,especially to understand and study the etiology and pathogenesis of blinding eye diseases,to understand the symptoms of each period,and to seek a suitable treatment.With the improvement of life,people pay more and more attention to eye health.Visual impairment and even blindness caused by ophthalmic diseases seriously affect the quality of life of patients.Many early and recurrent eye diseases first appear in the retina and choroid,such as glaucoma,diabetic retinopathy,retinal vein occlusion and other eye diseases,which seriously affect their health.It is important to detect these diseases in the retina and choroid fundus.Therefore,the study of the morphology,physiology and pathology of the retina and choroid in the fundus of the eye is of great practical significance to explore various ophthalmic diseases,especially to understand and study the etiology and pathogenesis of blinding eye diseases,to understand the symptoms of each period,and to seek a suitable treatment.Optical coherence tomography(OCT)plays an increasingly important role in biological imaging,especially in ophthalmic and cardiac blood flow imaging.It has been used in ophthalmology for a long time.Its resolution can reach microns,and the advantages of fast imaging speed and good repeatability make it very suitable for tomography imaging of translucent tissues such as eyes.At present,it is mainly used in the examination of posterior vitreous interface diseases,retinal and macular diseases,and pigment epithelial diseases.Photoacoustic imaging of ophthalmology is the recent development of a new type of technology,it is based on the principle of photoacoustic imaging,light to the eyes,blood vessels,pigment and other groups absorb light pulse heat expansion,which in turn produces mechanical wave by ultrasonic transducer is the acoustic signal reception,and then through data reconstruction of the position of the absorber,and size.Due to the unique optical absorption characteristics in biological tissues and the unique high contrast of photoacoustics,it can provide good information about the function and structure of the eye.For example,some special eye diseases such as diabetic retinopathy and age-related macular degeneration can provide changes in the ocular circulation system and a series of important parameters such as blood oxygen saturation through photoacoustic ophthalmology imaging.It has incomparable advantages in disease diagnosis compared with other optical imaging methods.Multimodal imaging can provide richer information about the structure and function of biological tissues,which is beneficial to the analysis of ophthalmic pathology and the detection and diagnosis of related diseases.Using double modal imaging system for in vitro tissue imaging,combined with the OCT image and PAM to provide information and the optical absorption of optical scattering can obviously separation body tissue layers and quantitative analysis of its regional structure,and then use optical clearing agent,through the system for its optical scattering and optical absorption information,further analysis of the refractive index changes,the organization The interaction between light and tissue.Optical clearing agent is an agent that changes the refractive index between tissues.Study on the choroidal layer by changing the refractive index of tissue with optical clearing agent based on bimodal photoacoustic microscopic imaging system.Considering the tolerance of the eye and the weak absorption and scattering of fundus light,we will obtain the structure and function imaging of biological tissues synchronously from the scattering characteristics and light absorption characteristics of living New Zealand white rabbits by retrobulbous injection of phototransparent agent.The multimodal imaging system can be used to observe and estimate the changes in the structure and function of the fundus tissues caused by retinal diseases,so as to achieve accurate early diagnosis of retinal diseases.