Prognostic Analysis Of Tumor Infiltrating Lymphocytes In Breast Cancer Based On Multiphoton Image

According to the latest global burden of cancer data for 2020,overtaking lung cancer with 2.2 million new cases worldwide as the number one cancer worldwide.Previous studies mainly discussed the mechanism of tumor genesis and development from the perspective of tumor itself,from the perspective of tumor microenvironment,exploring the interaction between tumor microenvironment and tumor cells has gradually become a research hotspot in recent years.The interaction between the immune system and cancer is complex,the interaction between immune cells and tumor cells is crucial for the development and progression of cancer.Tumor infiltrating lymphocytes(TILs),as a prognostic and predictive biomarker,have been reported in many breast cancer studies.Currently,all studies have analyzed TILs using hematoxylin-eosin staining,immunohistochemistry,tissue microarray(TMA),or gene expression profiling.Multiphoton microscopy(MPM)is a new advanced biomedical imaging method based on nonlinear optical effects.Optical signals generated by femtosecond laser interaction with biological tissue can be imaging at the cellular and molecular levels with submicron resolution.MPM has the advantages of depth imaging,matt bleaching,phototoxicity and real-time imaging.Currently,MPM has been widely used in scientific research in biology,medicine,materials science and other fields,and has great potential in clinical identification and diagnostic evaluation of tumors.The prognostic effect of multiphoton imaging on TILs is still unknown.Therefore,in this paper,multi-photon microscopy was used to obtain multi-photon image characteristics of tumor cells and lymphocytes,which laid a foundation for studying the prognostic effect of tumor infiltrating lymphocytes.In this paper,multi-photon microscopy was used to first study the imaging of TILs.According to the relative positions of lymphocytes,tumor cells and collagen fibers in multi-photon images,three lymphocyte distribution patterns TILs 1-3 were evaluated.Then we explored and determined the relationship between the distribution pattern of TILs and the status of lymph node metastasis,and then used the distribution pattern of TILs to predict histological grade.These studies provide new ideas for the study of breast tumors and make it possible to apply multi-photon imaging technology in the pathological diagnosis of breast cancer during surgery,which has great scientific research significance and biomedical application prospect.