Determination Of Serum 25(OH)D Levels In Patients Without Dialysis In Patients With Chronic Kidney Disease By Liquid Chromatography-tandem Mass Spectrometry And Its Correlation

Objective:To investigate the composition of serum 25（OH）D and the correlation between25（OH）D₃levels and laboratory indexes in patients without dialysis of chronic kidney disease CKD.Methods: A total of 89 patients with CKD who attended the Department of Nephrology of Shanxi Bethune Hospital from August 1,2022 to November 31,2022 were enrolled,and their e GFR levels were divided into CKD1-2 stage,CKD3-4 and CKD5 groups,and 12healthy adults in the physical examination center were selected as the control group. General clinical data and laboratory indicators were collected,liquid chromatography-tandem mass spectrometry was used to determine the levels of 25（OH）D₂and 25（OH）D₃,and the detection rate of 25（OH）D₂and the correlation between25（OH）D₃and other biochemical indexes were analyzed.Results:25（OH）D deficiency is prevalent in patients with CKD,and 25（OH）D₂may be a factor influencing the diagnosis of vitamin D deficiency.25（OH）D₃in the CKD group was significantly lower than that in the control group,and the CKD3-5 stage gradually decreased with the progression of the disease 25（OH）D₃,Spearman correlation analysis showed that regardless of healthy or CKD population,sex,Hb,and U-PRO were all associated with 25（OH）D₃（P<0.05）,ALB was significantly associated with 25（OH）D₃level（P<0.0001）,and in CKD population,Ca was significantly associated with 25（OH）D₃level（P<0.0001）,multiple linear regression suggests that the content of25（OH）D₃is mainly influenced by ALB.Conclusions:ALB in patients with CKD is closely related to the content of 25（OH）D₃,and the relationship between 25（OH）D₃and glycolipid metabolism and calcium and phosphorus regulation is weak.