Study On The Prognostic Value And Biological Function Of Long Non-coding RNA AC008063.3 In Head And Neck Squamous Carcinoma

Objective:Head and neck cancer(HNC)is the seventh highest incidence in the body,with over90% of cases being squamous cell carcinoma.Invasion and metastasis,along with chemoradiotherapy resistance,are major factors contributing to poor prognosis.It has been reported that long non-coding RNA(lnc RNA)is abnormally expressed in a range of tumors,including head and neck squamous cell carcinoma(HNSCC),and is related to the malignant phenotype of tumors,which opens a new direction for the research of HNSCC.This study aims to analyze the relationship between the expression level of the AC008063.3 and the clinicopathological parameters of patients with HNSCC,and to clarify the biological function of AC008063.3 in HNSCC cells.Moreover,it provides a potential biomarker for diagnosing,treating,and prognostically evaluating HNSCC.Methods:First,the expression levels of AC008063.3 in HNSCC tissues were analyzed using transcriptome sequencing data from The Cancer Genome Atlas(TCGA),and association with clinical parameters and prognosis of patients.The expression of AC008063.3 in HNSCC cell lines was detected using Real-time Quantitative PCR(q PCR).Secondly,the knockdown effects of AC008063.3 on the proliferation,invasion,metastasis,and apoptosis of HNSCC cells were detected using CCK-8,clone formation,Transwell,and flow cytometry assay.Finally,the AC008063.3 ce RNA network was constructed,and Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses were conducted to annotate the target messenger RNA(m RNA)of AC008063.3.Results:Long non-coding RNA AC008063.3 was significantly upregulated in HNSCC tissues and four cell lines.High AC008063.3 expression contributed to the poor overall survival rate(OS),disease-specific survival(DSS)and progression-free interval(PFI),as well as positively correlated with the pathological state.AC008063.3 downregulation significantly inhibited HNSCC cell proliferation,colony formation,migration and invasion,while promoting cell apoptosis(P < 0.05).The AC008063.3-centered ce RNA network includes 9micro RNA(mi RNA)nodes and 547 m RNA nodes.GO and KEGG analysis showed that AC008063.3 may function as an oncogene by regulating related biological processes and pathways.Conclusion:Long non-coding RNA AC008063.3 is highly expressed in HNSCC tissues and cell lines,and its expression level correlated with adverse pathological parameters,indicating its oncogenic role in HNSCC.AC008063.3 can significantly affect the proliferation,colony formation,invasion,and migration of HNSCC cells.Thus,AC008063.3 may serve as a potential biomarker for HNSCC.This study also provides important clues for revealing the downstream regulatory mechanism of AC008063.3.