| Objective: To investigate the effect of Yiren FUzi Baijiangsan(YFB)on DSS induced Ulcerative Colitis(UC)in mice.To study the effect of NF-κ B/NLRP3 pathway and related inflammatory factors on the prevention and treatment of UC in mice.Methods: 40 KM/SPF male mice fed freely for one week were randomly divided into 4 groups: normal group(Blank group),model group(DSS group),Coiren Fuzi Septicum SAN group(YYFZBJS group)and sulfasazine group(SASP group).Except for the normal group,the mice ulcerative colitis model was induced by 4%DSS aqueous solution for free drinking in other groups,and the modeling and administration were carried out simultaneously for 10 consecutive days.The weight,living status and feces of the mice were observed.DAI scores of the mice were calculated at the end of the experiment on the 17 th day.The mice were anesthetized,their blood was extracted by eye extraction,and serum was collected after centrifugation.Finally,the mice were killed by lifting the tail vertebra and colon specimens were collected,their length was measured,and the colon specimens were treated in the following two ways :1.After being fixed with formaldehyde for 48 hours,3 colon specimens were randomly selected from each group,and HE staining was performed,observed and photographed by microscope,and the related pathological changes of HE staining were analyzed by image system.2.Part of the colon tissue in the middle 1/3 of the remaining colon specimens were taken,and the expression levels of NF-κB,I-κ B,Caspase-1 and NLRP3 proteins in the colon tissues were detected by WB method.Result: Compared with the normal group,the activity of model group was slowed down,the response to external stimulation was reduced,the mice lay down and were unwilling to move,the diarrhea and watery stools were serious,the padding was wet and dirty,the gross bloody stools or anal adhesion and blood stains were found in severe cases,and the test of fecal ocellular blood was seriously positive(++++),the change rate of body mass was significantly decreased(P<0.01),and the back had obvious coolness.The hair was fluffy and scattered without regular loss of luster,DAI was significantly increased(P<0.01),colon length was significantly shortened(P<0.01),HE staining under the microscope showed a large number of inflammatory cell infiltration and large ulcers,and the serum pro-inflammatory factor content was found to be abnormally active by ELISA.TNF-α,IL-1 β,IL-6 and IL-17 were significantly increased(P<0.01),the content of anti-inflammatory factor was relatively insufficient,and IL-10 was significantly decreased(P<0.01).The expression of related proteins in the NF-κ B/NLRP3 pathway in colon was abnormally increased by WB assay.NF-κ B,Caspase-1 and NLRP3 were significantly increased(P<0.01),NF-κB inhibitory protein was relatively insufficient,and IκB was significantly decreased(P<0.01).Compared with the model group,the above weakness and general physiological status of mice in the coix seed and aconiti patrinia powder group were significantly improved,the change rate of body mass was significantly decreased(P<0.01),DAI was significantly decreased(P<0.01),and colon length was significantly increased(P<0.05).HE staining under the microscope showed that inflammatory cell infiltration was significantly reduced,and ulcer area was reduced.ELISA showed that the serum pro-inflammatory factor content was decreased,TNF-α,IL-1 β,IL-6 and IL-17 were significantly decreased,with statistical significance(P<0.01,P< 0.05),anti-inflammatory factor content was increased,and IL-10 was significantly increased(P<0.01).The expression of NF-κB/NLRP3 pathway in colon was significantly decreased by WB assay,and NF-κB,Caspase-1 and NLRP3 were significantly decreased,with statistical significance(P<0.01).The expression of NF-κB inhibitory protein was relatively increased,and I-κ B was significantly increased(P<0.05).Compared with the model group,the frailty of sulfasalazine group was significantly improved compared with the general physiological condition,but there was no significant difference compared with the coiren Fuzi Patrinia SAN group.Conclusion: 1.Coix Fuzi RPI Powder may reduce DSS induced ulcerative colitis by down-regulating the contents of serum IL-1β,TNF-α,IL-6 and IL-17 proinflammatory factors and up-regulating the contents of IL-10 anti-inflammatory factors in UC mice.2.Coix Fuzii Patrinia Powder may inhibit DSS induced ulcerative colitis in mice by regulating NF-κ B/NLRP3 pathway in cell pyrodeath,and is associated with down-regulation of NF-κB,NLRP3 and caspase-1,and up-regulation of IκB.3.Coix Fuzii Septicus Powder may regulate the content of inflammatory factors by regulating the NF-κB/NLRP3 pathway in cell pyrodeath to inhibit DSS induced ulcerative colitis in mice. |
