LncRNA TUSC7 Interferes With MiR-181a’s Study Of Osteosarcoma Progression By Sponge

Objective:This article aims to elucidate the regulatory effect of TUSc7 on osteosarcoma,and explore that TUSc7 interferes with miR-181a through sponge,thereby inhibiting the further development of osteosarcoma,and providing a new target for the clinical diagnosis and treatment of osteosarcoma.Method:(1)TUSc7 overexpression vector and control vector were transfected to MG63 and U2OS,respectively,and the overexpression level of TUSc7 in osteosarcoma cell lines U2OS and MG63 was detected by RT-qPCR.(2)CCK-8 experiment and colony formation experiment detected the proliferation ability of osteosarcoma when TUSc7 was overexpressed;(3)Transwell experiment observed cell migration and invasion between TUSc7 overexpression vector group and control vector group.(4)RT-qPCR experiments were used to detect the localization of TUSc7 in osteosarcoma cells;(5)The binding relationship between TUSc7 and miR-181a was predicted by Starbase database,and verified by diluciferase experiments;(6)The expression level of miR-181a detected by RT-qPCR was transfected with TUSc7 overexpression vector and TUSc7 empty vector into MG63 and U2OS cell lines.Results:(1)After the TUSc7 overexpression group was transfected with U2OS and MG63 cells,RT-qPCR analysis showed that the expression level of TUSc7 was significantly increased;(2)The results of CCK-8 and colony formation experiments showed that the cell proliferation rate and colony formation ability of the TUSc7 overexpression group were significantly lower than those in the control group.(3)Transwell experimental results showed that after TUSc7 overexpression,the migration and invasion of U2OS and MG63 cells were significantly reduced.(4)RT-qPCR showed that TUSc7 was mainly localized to the cytoplasm;(5)After transfection of miR-181a mimic,the luciferase activity of TUSC7-WT was significantly reduced,but the luciferase activity of TUSC7-Mut was not significantly reduced;The expression of miR-181a in OS tissues and cell lines increased significantly.(6)The expression level of miR-181a was detected by RT-qPCR in the TUSc7 overexpression vector and TUSc7 empty vector into MG63 and U2OS cell lines,and the expression level of miR-181a was significantly downregulated compared with the control vector.Conclusion:(1)TUSc7 overexpression inhibits the progression of OS.(2)TUSC7 competitively binds to miR-181a by sponge,inhibiting its expression in OS cells.