Studies On Chemical Constituents And Their Biological Activities Of Pittosporum Truncatum Pritz And Pittosporum Elevaticostatum Chang Et Yan

The species of secondary metabolites of Pittosporum are rich,including terpenoids and their saponins,phthalates,carotenoids,etc.Their pharmacological activities are mainly anti-tumor,anti-inflammatory,antibacterial and neuroprotection.In this paper,the chemical constituents of the branches and leaves of Pittosporum truncatum Pritz and the leaves and fruits of Pittosporum elevaticostatum Chang et Yan were extracted,separated,identified,and evaluated in their biological activity.Pittosporum truncatum Pritz is collected from Ziyuan town,Ziyuan county,Guilin city,Guangxi province.Pittosporum elevaticostatum Chang et Yan is collected in Li autonomous county,Changjiang city,Hainan Province.A total of fifty-two compounds were isolated and identified by various chromatographic techniques（normal phase silica gel column chromatography with different solvent systems,reverse phase C-18 column chromatography,Sephadex gel chromatography,HPLC and TLC detection etc.）and spectroscopic analysis(¹H-NMR、¹³C-NMR、DEPT、COSY、HSQC、HMBC、NOESY、HRESIMS、IR、UV、ORD、ECD),including nine new compounds and two new natural products.1.Eight compounds were isolated and identified from the branches and leaves of Pittosporum truncatum Pritz,including one new pyrrole alkaloid（1）and five glycoglycerolipids;1 and 5 showed significant nitric oxide production inhibition on LPS-induced BV-2 cells with IC₅₀values of 8.81 and 3.19μM,respectively.2.Thirty compounds were isolated and identified from the leaves of Pittosporum elevaticostatum Chang et Yan,including eight new compounds（9-13,24-26）;From a chemical structural,fifteen oleanane triterpenoids and ursane triterpenoid（9-23）,five polyenes and polyalkenes（24-28）and ten other compounds（29-38）.Fourteen compounds were isolated and identified from the fruits of Pittosporum elevaticostatum Chang et Yan,including two new compounds（39-40）;The isolated compounds showed abundant metabolites of Pittosporum.It enriches the chemical composition of Pittosporum and provides a theoretical basis for further rational development and utilization of plant resources.At the same time,some active compounds with anti-neuroinflammatory activity were found,providing important sources of possible lead compounds for the development of anti-neuritic drugs.