Effect Of TRIM21 On Proliferation And Apoptosis Of Acute Leukemia Cells

Objective:This study attempts to explore the effect of the differential expression of TRIM21 on the proliferation and apoptosis of acute leukemia cells by constructing the TRIM21 overexpression and knockdown models of acute leukemia cells,and to clarify the effect of TRIM21 on the occurrence and development of acute leukemia,so as to provide a new direction for the targeted therapy of acute leukemia.Methods:Two acute leukemia cell lines were selected,and the two cells were infected with lentivirus to construct the knockdown model,and the lentivirus transfection efficiency of the cells was observed under a fluorescence microscope.In order to detect the effect of TRIM21 knockdown in cells,the transcription level of TRIM21 was detected by PCR assay.The protein expression level of TRIM21 was detected by Western-bolt assay.In order to verify the effect of TRIM21 on the proliferation of acute leukemia cells,the cell proliferation of TRIM21 knockdown group,control group and overexpression group were detected,and the cell cycle of TRIM21 knockdown group was detected by flow cytometry.The apoptosis of TRIM21 knockdown group,control group and TRIM21 overexpression group were detected.Finally,the differential expression of TRIM21 in acute leukemia samples was retrieved by bioinformatics analysis for the regulation of signaling pathways and gene regulation,and it was found that the abnormality of TRIM21 would lead to the abnormality of P53.Finally,the expression of P53 was detected at the transcriptional and translational levels.Results:(1)After TRIM21 knockdown,the cell survival rate was significantly decreased,cell proliferation was inhibited,and cell apoptosis was increased.(2)TRIM21 knockdown induced cell cycle arrest at G0/G1 phase.(3)After TRIM21 overexpression,the survival rate of acute leukemia cells was greatly improved,and the cell apoptosis was reduced.(4)P53 expression was down-regulated in AL cells overexpressing lentivirus TRIM21.Conclusion:(1)After TRIM21 knockdown,the survival rate of acute leukemia cells was decreased,cell proliferation was blocked,and cell apoptosis was increased.(2)TRIM21 knockdown inhibited the proliferation of AL cells possibly by arresting the cell cycle at G0/G1 phase.(3)After TRIM21 overexpression,the survival rate of acute leukemia cells was greatly improved,and the cell apoptosis was reduced.(4)P53 expression was down-regulated after TRIM21 overexpression.