| The relationship between the health of the female reproductive tract and microorganisms is one of the focuses of human microbiome research.More and more studies have shown that female reproductive tract microorganisms are associated with many gynecological diseases and even cancer.Among them,persistent high-risk human papilloma virus(HPV)infection is the main cause of cervical cancer(CC).The relationship between the occurrence and development of CC and microorganisms may be traced back to the early stage of HPV infection.Understanding the impact of HPV infection on the microbial composition of the reproductive tract in asymptomatic populations has certain significance for the ultra early diagnosis of CC.Additional supplementation of probiotics with the ability to inhibit the growth of pathogenic bacteria can adjust the microbial community structure of the reproductive tract,making the reproductive tract environment more conducive to the growth of lactic acid bacteria,achieving the purpose of prevention and adjuvant treatment.This article studied the microbial composition of the cervix,vagina,urinary tract,and intestinal tract of healthy women of reproductive age from different ethnic groups in the same settlement,and explored the possible impact of HPV on the microorganisms in the reproductive tract and adjacent organs during the asymptomatic stage of infection;To explore the impact of HPV infection and microbial community on the host by studying the changes in subject related cytokines.A total of 103 subjects were collected for cervical exfoliation,vaginal,urethral,and rectal swabs,and their microbial communities(including HPV typing,bacterial,and archaea 16 S rRNA gene sequencing)were analyzed.Serum samples were collected from each subject to detect cytokine content.Overall,48% of women present HPV positive,with different proportions and types of HPV infection detected in cervical(14.56%),vaginal(33%),urethral(29.13%),and rectal(13.59%)swabs.HPV detected in vaginal swabs is usually a high-risk type,while low-risk HPV is mainly found in rectal swabs.This proportion varies among different ethnic groups.In cervical,vaginal,and urethral samples,Lactobacillus predominates,and its abundance decreases in HPV positive samples.Cytokines are associated with HPV infection and microorganisms in the reproductive tract.In this study,the levels of IL-2,IL-4,IL-6,and IL-10 in HPV positive sites were higher than those in HPV negative sites.Can clearly see IFN-γ And TNF-α There was a positive correlation with Gardner’s bacteria(P<0.5),but there was no significant negative correlation with Lactobacillus.Using MRS medium for isolation and culture of vaginal swabs,it was found that the bacteriostatic effect of Lactobacillus K6M5 isolated from the vagina was better than that of Enterococcus faecalis K2M2,but both were weaker than that of foodborne Lactobacillus.Foodborne lactic acid bacteria have a higher antibacterial spectrum and can inhibit most pathogenic bacteria including Candida albicans,while vaginal lactic acid bacteria cannot effectively inhibit fungal Candida albicans.This study is the first step in providing personalized medication for women’s reproductive health.Differences in the microbiome of the individual reproductive tract will be considered in risk assessment and subsequent disease diagnosis and treatment.At the same time,the differences in microorganisms among ethnic groups will provide ideas and basic data for future research.Further exploration and development are focused on lactobacillus,using probiotics for adjuvant treatment from a microbial perspective,and providing prevention and intervention measures in the early stages of disease. |
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