A Preliminary Study Of S100A4 In HaCat Cells In The Pathogenesis Of Psoriasis

Objective: To investigate the regulation of differentially expressed genes in HaCat cells on the pathogenesis of psoriasis,and to explore the influence of S100A4 gene expression patterns and pathways in variable splicing events on the pathogenesis of psoriasis,so as to provide new ideas for further understanding of the pathogenesis of psoriasis.Method: In HaCat cell beads,passage culture was carried out,transcriptional sequencing technology(RNA-seq)was used to study the effects of silencing S100A4 on gene expression pattern,expression level and variable splicing events at the overall level,and differential expression gene analysis of samples was carried out using edg R software package.ABLas software was used to systematically analyze the splicing types of each sample and analyze the influence of transcriptome data changes.Real-time fluorescence quantitative PCR(RT-Q-PCR)was used to verify the silencing efficiency of S100A4.Bioinformatics was used to analyze cell proliferation,cell cycle,keratinocyte differentiation and angiogenesis.Results: The results of RT-Q-PCR showed that S100A4 was successfully knocked down.Illumina Novaseq6000 was used to sequence the constructed library,and high quality transcriptome data were obtained.The average base quality Q30 was 95.40%.The volcano map showed that there were 747 differentially expressed genes caused by silencing S100A4,among which 345 up-regulated genes and 402 down-regulated genes,indicating that S100A4 extensively regulated gene transcription in HaCat cells.S100A4 can regulate the variable splicing events of the genome in HaCat cells,with 769 up-regulated and904 down-regulated differential variable splicing genes.The GO enrichment pathway list showed several functional pathways related to apoptosis and cell cycle.Conclusion: S100A4 is related to psoriasis cell proliferation and angiogenesis according to functional enrichment analysis.The regulation of S100A4 in HaCat cells was studied by analyzing variable splicing events.The variable snips of IFI16,HUS1,MPRIP,MIR205 HG,VEGFA and DDR1 genes are regulated by S100A4,indicating that S100A4 plays an important role in inflammation and angiogenesis.