| Objective: To explore the association between the Single-nucleotide polymorphism of A disintegrin and metalloproteinase with thrombospondin motifs 7(ADAMTS7)gene rs6201102,rs62012628 and essential hypertension susceptibility.Methods: Two hundred and eleven cases of essential hypertension were selected as the case group and208 cases of normal blood pressure in the same period of physical examination were selected as the control group,and general data and blood specimens were collected from the case and control groups.DNA was extracted from all study subjects in the case and control groups,and the target fragments of rs62011052 and rs62012628 loci were analyzed for genotype by PCR amplification followed by Sanger sequencing.SPSS25.0 software was selected to analyze and process the study data.t-test or Mann-Whitney U-test was used to analyze the differences in measures between the case and control groups,χ2 test was used for Hardy-Weinberg equilibrium test and comparison of gene frequencies between the two groups,and logistic regression model was used to analyze association of different genetic models at the rs62011052 and rs62012628 loci with susceptibility to essential hypertension disease,GMDR was used to explore ADAMT7 gene-environment interactions.Results:(1)The differences in the distribution of body mass index(BMI),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),total bile acids(TBA),uric acid(UA),and resting he art rate(RHR)between the hypertensive group and the control group were statistically significant(P<0.05);the differences in the distribution of age,smoking history,alcohol history,total cholester ol(TC),low-density lipoprotein cholesterol(LDL-C),and fasting blood glucose(FBG)distribution were not statistically significant(P>0.05),and multi-factor unconditional logistic regression analysis:RHR(OR=1.071,95% CI:1.045-1.098,P<0.001),BMI(OR=1.241,95%CI:1.145-1.344,P<0.001),a nd TG(OR=1.301,95%CI:1.018～1.663,P=0.035)were risk factors for hypertension.(2)The genotyp e distribution of rs62011052 and rs62012628 loci between the two groups was consistent with Har dy-Weinberg equilibrium(P>0.05).(3)The differences in genotype frequencies and allele frequenci es at the rs62011052 and rs62012628 loci were not statistically significant between the two groups(P>0.05).The differences remained statistically insignificant when the two groups were analyzed a fter stratification according to sex(P>0.05).(4)After adjusting for confounding factors such as his tory of smoking,history of alcohol consumption,FBG,BMI and blood lipids in the analysis,no s ignificant correlation was seen between people carrying different genotypes at the rs62011052 and rs62012628 loci and the risk of developing hypertension under different genetic models(P>0.05).(5)The differences in blood pressure values between genotypes under different genetic models wer e not statistically significant at rs62011052,and the differences in DBP between genotypes under t he co-dominant model were statistically significant at the rs62012628 locus(P<0.05),followed by a two-by-two comparison,with the DBP of genotype CT lower than that of genotype CC in the c o-dominant model(P<0.05);in dominant model,SBP and DBP were lower in the CT+TT genotyp e than in the CC genotype(P<0.05),and the differences in blood pressure values between the rem aining genotypes under different genetic models were not statistically significant(P>0.05).(6)The GMDR analysis showed that the one-way model(BMI)had the greatest cross-consistency and stati stically significant differences(P<0.05)and was the best model.Conclusion:(1)Polymorphism at the rs62011052 locus of the ADAMTS7 gene was not significantly associated with genetic susceptibility to essential hypertension;(2)The T allele at the rs62012628 locus of the ADAMTS7 gene is associated with lower blood pressure value levels.(3)There was no interaction between the ADAMTS7 gene rs62011052,rs62012628 loci and various environmental factors in the development of essential hypertension. |
