| Objective: This study used bioinformatics technology to integrate relevant gastric cancer(GC)datasets into the Gene Expression Omnibus(GEO)database to identify differentially expressed lnc RNAs in GC tissues and corresponding paracancerous tissues,clarify the expression levels of LINC00342 in GC tissues and cells and its effects on GC cell functions and predict the miRNAs regulated downstream of LINC00342 to provide more relevant targets for studies investigating the screening,prevention,and treatment of GC.Methods: Using bioinformatics techniques,lnc RNAs and their downstreamregulated miRNAs were identified through screening and literature search using the GEO database and related prediction software.q RT-PCR was used to analyze the differential expression of LINC00342 and miR-596 in GC cell lines,human gastric mucosal cells,GC tissues,and the corresponding paracancerous tissues.GC BGC-823 cell line was transfected with the following: LINC00342-overexpressed plasmid and a corresponding negative control;miR-596 mimic and corresponding negative control.GC MGC-803 cell line was transfected with the following: si RNA interference LINC00342 and a corresponding negative control;miR-596 inhibitor and corresponding negative control.The assays of Edu cell proliferation,CCK-8,wound healing,transwell cell invasion,and cell cycle were done to validate the impact of LINC00342 and miR- 596 on the function of GC cells.Lastly,LINC00342 and miR-596’s targeting regulatory interaction was investigated utilizing a luciferase reporter assay.Results: The lnc RNA studied was finally identified as LINC00342 via bioinformatics techniques and prediction analysis using a related software,and the miRNA predicted to be regulated downstream was miR-596.The LINC00342 expression level in GC tissues and human gastric mucosal cell lines was greater than those matched adjacent tissue and human GC cell lines(all P<0.05)).Contrastingly,the miR-596 expression level in GC tissues and human gastric mucosal cell lines was lower than those matched adjacent tissue and human GC cell lines(all P<0.05)).On the one hand,overexpression of LINC00342 enhanced proliferation(P<0.05),migration(P<0.01),and invasion(P<0.001)while suppresed the proportion in G0/G1 phase cells(P<0.01).In contrast,knocking down LINC00342 suppressed cell proliferation(P<0.05),migration(P<0.01),and invasion(P<0.001)while increasing G0/G1 phase cells proportion(P<0.01).Interestingly,miR-596 played the opposite role.LINC00342 served as a sponge of miR-596(P=0.0067).Conclusion: 1.LINC00342 was expressed higher in gastric cancer tissues than in paraneoplastic tissues and higher in human gastric cancer cells than in human gastric mucosal cells,while the opposite was true for miR-596.2.LINC00342 promotes the proliferation,migration and invasion of gastric cancer cells through the regulation of miR-596. |
