The Research Of Chemical Components From Pheretima Aspergillum(E.Perrier) And Populus Euphratica

Objective : Pheretima aspergillum(E.Perrier)was first recorded in the monograph Shennong’s Classic of Materia Medica.It has the effect of clearing heat and calming shock,clearing collaterals and relieving asthma and diuresis.Populus euphratica is known as the "hero of the desert" and distributed in western Inner Mongolia,Gansu,Xinjiang and other places.It is widely used to treat tonsillitis,duodenal ulcer and pain.This study mainly focused on the chemical constituents of Pheretima aspergillum(E.Perrier)and Populus euphratica,aiming to further explore their chemical structure types and screen out bioactive components,so as to provide scientific reference for the full development and utilization of medicinal resources of Pheretima aspergillum(E.Perrier)and Populus euphratica and clarify their medicinal material basis.Methods : The total extract of Pheretima aspergillum(E.Perrier)and Populus euphratica was separated by repeated column chromatography methods of MCI gel CHP 20 P,RP-18,Sephadex LH-20,silica gel column chromatography,HPLC and other materials,and combined with the technical guidance of LC-MS/DAD and a variety of spectroscopic methods to separate the compounds,and the structures of the compounds was solved and identified by spectroscopy method and ECD calculation method.The anti-inflammatory activity of some compounds was evaluated by CCK-8.Results:A total of 33 compounds were isolated and identified from the two traditional Chinese medicines.21 compounds were isolated from the Pheretima aspergillum(E.Perrier)and 12 compounds were identified from the Populus euphratica,including 6 new compounds.The results showed that compounds 25 and 27 had good anti-inflammatory activity at 40 μM.Conclusion: The above findings enrich the diversity of small molecular material structure of Pheretima aspergillum(E.Perrier)and Populus euphratica,and provide a basis for elucidating their pharmacodynamic substances.The bioactivity findings will help explain the traditional medicinal efficacy of Populus euphratica and provide a lead compound for the development of anti-inflammatory drugs.