Research On Treatment Of Osteoarthritis Basedon Polymer Microneedle Therapy

Osteoarthritis has become the most common chronic disease among middle-aged and elderly people in China.The typical symptoms of patients with osteoarthritis are joint pain,joint swelling,stiffness,and so on.It can cause stiffness of the knee joints,inconvenience in movement,inability to squat,and even inability to walk and climb stairs normally,seriously affecting daily life.As a traditional Chinese medicine treatment method for treating osteoarthritis,people have been paying close attention to it.However,the limitations of its drug delivery methods have led to a significant reduction in the therapeutic effect of drugs,and subsequently brought about secondary diseases,reducing patient compliance.The development of transdermal drug delivery systems provides new possibilities for the delivery of traditional chinese medicine.As a new type of percutaneous drug delivery,microneedle has attracted widespread attention.This article focuses on the study of percutaneous delivery efficiency of microneedle drugs.Firstly,by simulating the percutaneous absorption process of drugs,a material transfer diffusion model based on Fick diffusion law was established.Using Fick’s second law as the control equation and introducing the general initial conditions for percutaneous absorption,a single layer diffusion model was established,and numerical simulation was performed using MATALAB R2014 a to obtain the drug concentration distribution rules at different locations and at different times.Secondly,using polylactic acid as the substrate,PLA microneedles were prepared by micro injection.The appearance,penetration performance,and in vitro drug delivery efficiency of PLA microneedle patches were characterized.The experimental results showed that the needle height was 520 μm PLA microneedles have sufficient mechanical properties to pierce the stratum corneum of mouse skin.In the microneedle pretreatment and non pretreatment Franz diffusion experiments,120 minutes later,the absorbance value of the former traditional chinese medicine solution of Gubitongfang was 1.087,and the latter was 0.090.After linear fitting,the drug concentration in the microneedle pretreatment experimental group was at least 10 times that of the untreated control group.Finally,a soluble microneedle patch containing the total flavonoids of rhizoma drynariae was prepared using HA and PVP as the substrate.The appearance,penetration,dissolution,skin recovery,and in vitro drug delivery efficiency of the microneedle were characterized.The experimental results showed that the microneedle patch had sufficient mechanical strength,uniform mechanical curve changes,and no faults were present.It could be rapidly dissolved within 20 minutes,and the skin after being punctured could recover to its original state after 10 minutes,In the microneedle pretreatment and non pretreatment Franz diffusion experiments,after 180 minutes,the drug concentration in the microneedle pretreatment experimental group was at least three times that of the untreated control group.