| Objective: To investigate the effects of different wettable titanium surfaces prepared by Cold Atmospheric Plasma(CAP)on the activity,migration,adhesion and angiogenesis of Vein Endothelial Cells(VECs),and to explore the possible mediators of the optimal wettable titanium surface on the angiogenesis of VECs.To explore the possible mediators of VECs angiogenesis on the surface of optimally wetted titanium sheets and to provide a theoretical basis for promoting early osseointegration and improving implant success.Methods: 1.Four sets of titanium surfaces with different wettability were prepared by CAP.2.The contact angles of the four sets of titanium surfaces were measured by contact angle measurement,the surface morphology of the four sets of titanium surfaces was observed by scanning electron microscopy,the roughness of the four sets of titanium surfaces was measured by optical measurement,and the elements of the four sets of titanium surfaces were analysed by X-ray energy spectrometry.3.The morphology of VECs on the four sets of titanium surfaces was observed by inverted fluorescence microscopy.The activity and migration rate of VECs on the surface of the four groups of titanium sheets were compared by CCK8 and scratch assay,and the angiogenesis assay was performed to detect the angiogenic ability of VECs on the surface of the four groups of titanium sheets.4.The activity and migration rate of VECs on the surface of the four groups of titanium sheets were measured by Western-Blot,The expression levels of VEGFA and Vascular Endothelial Growth Factor Receptor 2(VEGFR2)were measured by Western-Blot,and the secretion levels of VEGFA on the surface of the four titanium sheets were measured by Elisa.SPSS 22.0 software was used for statistical analysis of the data.Results: 1.Four groups of titanium flake surfaces with different wettability were prepared by CAP,and the contact angles were a(superhydrophilic group): 7±2°,b(hydrophilic group 1): 55±3°,c(hydrophilic group 2): 85±5°,and d(hydrophobic group): 128±7°.2.The content of carbon on the surface of titanium flakes was the lowest in the superhydrophilic group,and the content of carbon gradually increased with the increase of contact angle(P< The surface roughness Ra of the four groups was 2±0.06μm,2±0.40μm,2±0.13μm and 2±0.40μm,and the differences were not statistically significant(P>0.05).3.The early adhesion and activity of VECs on the surface of titanium flakes in the superhydrophilic group were the strongest,and the migration rate was significantly faster than that of the other groups,and as the contact angle increased,the adhesion,activity and migration ability of VECs gradually decreased(P<0.05).The VEGFA and VEGFR2 protein expression levels were the highest on the surface of titanium sheets in the superhydrophilic group,and the larger the contact angle,the lower the protein expression of VEGFA and VEGFR2(P < 0.05).,the difference was statistically significant(P < 0.05).Conclusion: 1.VECs showed the highest activity,migration,adhesion and angiogenic ability on the surface of superhydrophilic titanium sheets.2.Superhydrophilic titanium sheets may promote the angiogenesis of VECs by upregulating the expression of VEGFR2 on the surface of VECs. |
