Study On The Mechanisms Of Regulatory B Cells (B-regs) In The Chronicity Of Brucellosis

Objective:To study and analyze the changes of regulatory B cells（B-regs）and related cytokines（IL-10,IFN-γ）in patients with brucellosis at different stages of disease,understand the role of B-regs in the immune response of patients with brucellosis,and explore the role of regulatory B cells（B-regs）in the mechanism of chronic brucellosis.Methods:This study was prospective.A total of 30 patients with brucellosis in the acute and chronic stage were separately collected from September 2021 to April 2022who were treated in the First and Eighth Affiliated Hospitals of Xinjiang Medical University,and 30 healthy control groups were established.The general data of the three groups were collected.Flow cytometry was used to detect the levels of CD19⁺CD24^hiCD27⁺B-regs in peripheral blood before and after treatment in all patients with brucellosis,and serum IL-10 and IFN-γlevels were measured by ELISA,compared with 30 healthy control groups,and their change characteristics and correlations were analyzed by statistical methods.Result:1.There was no statistically significant difference in gender and age among the three groups（P>0.05）。2.The level of B-regs in the three groups were compared,and the acute and chronic phase groups were significantly higher than the healthy control group before treatment,and decreased after treatment,but the chronic phase group was still higher than that of the healthy control group and the acute phase group,and the difference was statistically significant（P<0.05）.3.The cytokine test results showed,IL-10 levels were significantly higher in the acute and chronic phase groups before treatment and higher than those in the healthy control group,and the levels decreased in both groups after treatment and were still higher than those in the healthy control group,and the differences were statistically significant（P<0.05）.Correlation analysis showed a positive correlation between IL-10 and B-regs levels（P<0.05）.IFN-γlevels were significantly higher in the acute and chronic phase groups before treatment than in the healthy control group,and tended to become lower after treatment,and the difference was statistically significant（P<0.05）.Conclusion:Human infection with Brucella abortus is accompanied by high levels of IFN-γand B-regs are elevated in different disease stages of brucellosis,while positively correlating with IL-10 levels.Patients in the acute phase have significantly increased B-regs levels and elevated IFN-γlevels,which can decrease after treatment,suggesting a good prognosis.Patients in the chronic phase often showed a significant increase in B-regs and low IFN-γlevels,and the detection of B-regs can provide an important reference value for the diagnosis and prognosis of the chronic phase of clinical brucellosis.