| Objective To investigate the serum levels of vitamin D and folic acid in children with autism spectrum disorder(ASD),compare them with normal children,and analyze the correlation between serum levels of vitamin D and folic acid in children with ASD and age,sex,severity of disease and developmental level.It provides a new idea for the treatment of children with ASD.Methods According to the DSM-Ⅴ,the diagnostic criteria for autism spectrum disorders were revised.A total of 114 children diagnosed with ASD for the first time in the Pediatric Rehabilitation Department of Anhui Children’s Hospital from March 2021 to June 2022 were selected as the ASD group,and healthy children matched with the ASD group in age and sex distribution during the same period were selected as the normal group.Chemiluminescence method was used to detect the levels of 25(OH)D and folic acid in the serum of children in the two groups,and ng/ml was used to represent the concentration unit.The differences of the levels of 25(OH)D and folic acid in the serum of the two groups were compared.The Childhood Autism Rating Scale,CARS)and Griffiths Developmental Scale-Chinese Version(GDS-C)were used to evaluate the severity and developmental level of children with ASD.The serum vitamin D and folic acid levels of children with ASD were evaluated in relation to age,sex,severity of disease and developmental level.Results 1.There were no significant differences in gender and age between the ASD group and the normal group(x2=0.107;t=-0.183,P>0.05);2.The level of serum 25(OH)D and folate in ASD group was 33.28±11.05 ng/m L compared with that in normal group(44.71±6.32)ng/m L.The ratio of(14.74±4.84)ng/m L to(23.17±6.16)ng/m L in ASD group was significantly lower than that in normal group,the difference was statistically significant(t=-9.113;t=-11.190,P<0.001);3.In boys,the levels of serum 25(OH)D and folate in ASD group and normal group were(33.81±11.31)ng/m L compared with(44.13±6.38)ng/m L.(14.93±4.80)ng/m L vs.(21.78±5.50)ng/m L,the difference was statistically significant(t=-7.483;t=-8.652,P<0.001);The ratio of serum 25(OH)D and folic acid between ASD group and normal group was(32.22±9.71)ng/m L compared with(46.74±5.77)ng/m L.(14.00±5.04)ng/m L vs.(28.07±5.97)ng/m L,the difference was statistically significant(t=-6.125;t=-8.562,P<0.001);4.Comparison of serum 25(OH)D and folic acid levels between boys and girls in ASD group:(33.22±11.45)ng/m L vs.(34.54±9.04)ng/m L;The ratio of(14.69±4.75)ng/m L to(14.96±5.29)ng/m L was not statistically significant(t=-0.515;t=-0.235,P>0.05);5.Age in ASD group was negatively correlated with serum 25(OH)D and folic acid levels(r=-0.273;r=-0.233,P<0.001)There was no significant correlation between age and serum 25(OH)D and folic acid levels in control group(r=0.024;r=0.030,P>0.05);6.Serum 25(OH)D and folic acid levels in ASD group were negatively correlated with CARS scores(r=-0.630;r=-0.567,P < 0.001),was positively correlated with GDS-C score(r=0.452;r=0.393,P < 0.001).Conclusion The serum levels of vitamin D and folic acid in ASD children are lower than those in normal children of the same age,and there is a correlation with age,severity of disease and development level,but no significant correlation with sex.There was no correlation between serum 25(OH)D and folic acid levels and age in normal children. |
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