Design Of Electric Field Generation System For Inhibiting Cancer Cell Proliferation

Cancer cells are essentially mutated cells with the characteristics of unlimited proliferation,convertibility and easy metastasis,which is the most fundamental cause of cancer.Cancer is a proliferative disease with strong invasiveness and rapid growth,and it is one of the public health security issues that seriously threaten human life and health.Traditional cancer treatment methods are mostly based on surgery,radiotherapy,and chemotherapy,which have relatively large side effects on the human body.However,the new electric field therapy technology can target and inhibit cancer cells instead of normal cells,which has little toxic and side effects on patients and does not affect normal tissues and organs,showing broad application prospects in cancer treatment.However,there are few reports on the detailed development process of related equipment.Therefore,the design of electric field generation system for inhibiting cancer cell proliferation is crucial.This thesis mainly focuses on the design of electric field generation system for inhibiting cancer cell proliferation and the research on the biological experiment of using this system to inhibit the proliferation of cancer cells.The specific research contents are as follows:In this thesis,based on the principle of alternating electric field generation and various amplifier background theories of analog electronic technology,the overall framework for the design of the electric field generation system for inhibiting cancer cell proliferation is determined.An Output Transformer Less power amplifier circuit and a Bridge-Tied Load operational amplifier circuit were designed respectively to realize the development of a high-voltage amplifier module.At the same time,design and manufacture the electrodes for electric field generation.Using the COMSOL Multiphysics finite element simulation software to simulate and calculate the electric field distribution in the cell culture medium under the action of the electric field electrode,and use the electric field detector to compare and verify the actual measured electric field intensity with the simulation results,which realized the design of electric field generation system for inhibiting cancer cell proliferation.Finally,based on the developed electric field generation system,a cell biology experiment was carried out with breast cancer cell MCF-7 as the experimental object.By setting up the control group and the electric field experiment group for comparison,the proliferation of breast cancer cells was analyzed.The results showed that the cells inhibition rate was 27.5%,which verified the effectiveness of this system in inhibiting the proliferation of cancer cells.The effects of alternating electric fields with different field intensity,frequency and waveform on the proliferation of cancer cells were further analyzed.Considering that other types of cancer cells also have the characteristics of cell mutation and uncontrolled proliferation similar to breast cancer cell MCF-7,this system also has guiding significance for the treatment of other types of cancer cells.In this thesis,the design of electric field generating system for inhibiting cancer cell proliferation is realized through the design of the high-voltage amplifier and the design of the electric field electrodes.The frequency of the output signal of this system is 100 k Hz-500 k Hz,the output voltage is about 100 V,any waveform including sine wave,triangle wave and square wave can be generated,and the electric field intensity is 0-5 V/ cm.In addition,this thesis uses the self-developed electric field generation system to reaserch the effect of breast cancer cell proliferation,verifing the effectiveness of this system on cancer cell inhibition,and basically clarifies the influence of various electric field parameters on cancer cell proliferation.