Studies On The Quality Standards Of Qiang Medicine Guangguoyou And Jiasuanjiang

Qiang medicine is an important part of traditional medicine in China.Guangguoyou is the whole plant of Caryopteris tangutica Maxim.Jiasuanjiang is a dried whole herb of Nicandra physaloides（L.）Gaertn.After research,Guangguoyou and Jiasuanjiang are used in Qiang medicine,but they are rarely recorded in existing written texts,and there are no systematic quality control standards.Objectives:（1）To study the quality standard control items of Guangguoyou and Jiasuanjiang in expectation of establishing preliminary quality standards for Guangguoyou and Jiasuanjiang,and to provide research support for subsequent studies and the standardized use of Guangguoyou and Jiasuanjiang.（2）To investigate the role of Guangguoyou in lipopolysaccharide（LPS）-induced inflammation model of RAW264.7 cells and to clarify the anti-inflammatory effect of Guangguoyou in an in vitro cellular inflammation model.METHODS:We reviewed existing literature and canonical texts,visited and researched Qiang doctors and Qiang medicine prescriptions,compiled the real-life applications of Guangguoyou and Jiasuanjiang,and determined the names of the herbs.The herbs of Guangguoyou and Jiasuanjiang were collected and identified,and the herbs were described for their properties,microscopic identification and thin-layer identification.The various examination items and leachate items of the herbs of Gangguoyou and Jiasuanjiang were examined and the corresponding limits were specified.Determine the appropriate methods for the determination of the content of the herbs of Gangguoyou and Jiasuanjiang,and perform the determination of the collected herbs and stipulate the limits.With reference to the literature and field research,we initially determined the herbs’properties,taste and meridians,functions and main treatment,usage and dosage.A lipopolysaccharide （LPS）-induced inflammation model for RAW264.7 cells was established.The concentration of Gangguoyou extract administration was determined by pre-experiment.The cellular activity was evaluated by CCK-8 method.The levels of nitric oxide（NO）release,mouse interleukin 1β（IL-1β）,mouse interleukin 6（IL-6）,mouse tumor necrosis factorα（TNF-α）and other cellular inflammatory factors were measured to investigate the effects of Guangguoyou extract on the inflammation model of LPS-induced RAW264.7 cells.RESULTS:Ten batches of Jiasuanjiang samples and five batches of Guangguoyou samples were collected.Guangguoyou and Jiasuanjiang were identified as the names of the herbs.The character description,identification,examination,content determination,and leachate of Guangguoyou and Jiasuanjiang were completed.Provisionally,the moisture content of Guangguoyou should not exceed 12.0%,the total ash content should not exceed 5.0%,and the acid insoluble ash content should not exceed 2.0%.Alcohol soluble leachate shall not be less than 16%.Provisionally formulated Jiasuanjiang moisture shall not exceed 13.0%,total ash shall not exceed 14.0%,acid insoluble ash shall not exceed 2.0%.Water-soluble leachate shall not be less than 20.0%.The method is simple,stable and reproducible.It is tentatively stipulated that the content of myricetin in Guangguoyou should not be less than 1.5%.Total flavonoids were determined as the content determination item of Jiasuanjiang,and it was provisionally stipulated that the total flavonoids in Jiasuanjiang should not be less than 1.5%in terms of rutin(C₂₇H₃₀O₁₆).Containing chlorogenic acid(C₁₆H₁₈O₉)shall not be less than 0.064%.The lipopolysaccharide-induced inflammation model of RAW264.7 cells was successfully established,and the concentrations of the Guangguoyou extract were 0.3 mg/ml,0.6 mg/ml,and1.2 mg/ml.The degree of inhibition of NO release was enhanced sequentially with increasing concentration of the drug.The results of ELISA showed that the secretion of IL-1β,IL-6 and TNF-αinflammatory factors from inflammatory macrophages was inhibited in all experimental groups,and the degree of inhibition of IL-1βand IL-6 inflammatory factors secretion increased with the increase of drug concentration.CONCLUSION:The preliminary quality standards of Guangguoyou and Jiasuanjiang were established.Guangguoyou extract can inhibit the release of IL-1β,IL-6 and TNF-αinflammatory factors and exert anti-inflammatory effects in a lipopolysaccharide-induced RAW264.7 cell inflammation model.