Research Of YTHDF3 Expression And Function In Colorectal Cancer

Background:Colorectal cancer(CRC)is one of the most common malignant tumors,and its morbidity and mortality are on the rise in China,bringing a huge burden to society and economy.increasing evidence indicates that the occurrence and progression of CRC is a complex multi-step process involving multiple levels of gene transcription and post-transcriptional regulation.In recent years,studies have found that N6-methyladenosine(m6A)is one of the most common post-transcriptional modification regulation of messenger RNA(mRNA),and plays an important role in the occurrence and development of tumors.However,there are few relevant studies on the function of YTHDF3 in CRC which is one of the core genes regulating m6A modification.Objective:This study aimed to clarify the expression level and clinical significance of YTHDF3 in colorectal cancer,and to preliminarily explore its ability to regulate CRC proliferation and migration.Methods:Immunohistochemistry(IHC)was used to detect the expression level of YTHDF3 in CRC tissue and its paired normal colorectal mucosal tissue,and to evaluate the correlation between its expression level and clinicopathological characteristics.The effect of down-regulation of YTHDF3 expression on the proliferation ability of CRC cells was studied by cytotoxicity experiments,plate cloning experiments and EDU experiments.The effect of down-regulating the expression level of YTHDF3 on the migration ability of CRC cells was studied by migration and scratch experiments.The effect of downregulating the expression level of YTHDF3 on the tumorigenic ability of CRC cells in vivo was studied by in vivo tumorigenic experiments in nude mice.Results:1.YTHDF3 was highly expressed in CRC,and its expression was related to the degree of tumor pathological differentiation(p=0.035),pathological stage(p=0.015)and distant metastasis(p=0.043)of CRC patients;2.Down-regulated YTHDF3 expression level Significantly inhibited the proliferation(All p<0.01)and migration(All p<0.05)abilities of CRC cells;3.The growth rate of subcutaneous xenograft tumors in nude mice with down-regulated YTHDF3 was significantly slowed down,and the tumor volume and weight were significantly reduced(All p<0.05).Conclusion:1.YTHDF3 is highly expressed in CRC,and is related to the degree of tumor pathological differentiation,pathological stage and distant metastasis of CRC patients;2.YTHDF3 promotes the proliferation and migration of CRC cells.