The Expression And Role Of NFE2L3 In Glioma

Glioma is the most common primary intracranial tumor,which is characterized by a high malignant degree,high postoperative recurrence rate,and drug resistance to chemotherapy.The current treatment methods are difficult to cure glioma,so more effective treatment of glioma has become a research hotspot.Nuclear factor erythroid2-like 3(NFE2L3),also known as NRF3,was first discovered in 1999 and is a member of the cap "n" collar(CNC)base leucine zipper family,a very important transcription factor.It plays an important role in regulating cell proliferation,metastasis,apoptosis and angiogenesis.In recent years,more and more cancer studies have confirmed this.NFE2L3 gene changes have been found in pancreatic,liver and colorectal malignancies.The NFE2L3 gene is a transcription factor involved in many pathways that regulate and control the malignant development of tumors.Therefore,the NFE2L3 gene may play a critical regulatory role in tumors.However,the role of the NFE2L3 gene in glioma remains unclear,and the specific molecular mechanism of whether the NFE2L3 gene can promote the occurrence and development of glioma needs to be further studied.In this study,the tumor Genome Atlas(TCGA)database,the Chinese glioma Genome Atlas(CGGA)database,the gene expression profile interaction analysis(GEPIA)database and the Human Protein Atlas(HPA)database were used to determine the expression level of NFE2L3 in all levels of glioma and its relationship with the prognosis of glioma.In order to explore the role of NFE2L3 in glioma,NFE2L3-si RNA vectors were constructed and transfected in glioma U251 and T98 cell lines.Real-time quantitative PCR and Weston Blot were used to detect the expression levels of NFE2L3 and MMP-9 in glioma U251 and T98 cells transfected with NFE2L3-si RNA.Cell scratch assay and transwell migration assay were used to detect the effect of NFE2L3 on the migration function of U251 and T98 cells.R language correlation analysis was used to obtain genes positively correlated with NFE2L3,and real-time fluorescence quantitative PCR was used to identify the relationship between the NFE2L3 gene and its positively correlated genes.This study found that NFE2L3 was highly expressed in human glioma tissues,and the high expression of NFE2L3 was associated with poor prognosis of glioma patients.NFE2L3 knockdown significantly inhibited the m RNA and protein expression levels of MMP-9 in U251 and T98 cells.NFE2L3 knockdown significantly inhibited the migration ability of U251 and T98 cells.In the CGGA database,NFE2L3 is positively correlated with CD58,CASP6,RIPK1,PHTF1 and FAM111A;in the TCGA database,NFE2L3 is positively correlated with ADAMTSL4,DPYD,HOXA1,PDPN and PTPN22.Knockdown of NFE2L3 can lead to decreased expression of HOXA1 at the m RNA level.The results of this study indicate that NFE2L3 can promote the migration of glioma U251 and T98 cells,and NFE2L3 can be used as a potential target for the clinical treatment of glioma.