Construction Of Bionic Magnetoelectric Nano-drug-carrying Platform And Application In Auxiliary Intervention Of Parkinson’s Disease

Objective:Parkinson’s disease(PD)is the second most frequent neurodegenerative disease after Alzheimer’s disease,and the aggregation of natural unfolded protein α-synuclein(α-syn)is the key to the occurrence and development of PD.Many therapeutic schemes aim at eliminating the aggregated α-syn or inhibiting its formation,but the elimination of α-syn is still a problem worth exploring.In addition,the degeneration of dopaminergic neurons leads to the decrease of dopamine(DA)synthesis.Here,we have developed a simple and effective strategy to realize the intervention of Parkinson’s disease through drug-loaded shell nuclear magnetic nano-materials coated with nerve cell membrane.Method:CFO nanoparticles coated with oleic acid were synthesized by improved precursor thermal decomposition method,and BCFO with core-shell structure was prepared by chemical coprecipitation and sol-gel reaction.BCFO@LCM was prepared by combining therapeutic drug L-DOPA and extracted cell membrane fragments on the surface of nanoparticles by electrostatic force,and its physical and chemical properties were characterized.After verifying its good biocompatibility in vitro,BCFO@LCM was applied to the intervention of Parkinson’s cells and mouse models and the therapeutic effect was evaluated.Results:BCFO@LCM showed excellent performance in drug release in vitro,stability,biological safety and therapeutic effect in vitro and in vivo.BCFO@LCM can protect nerve cells from MPP+-induced neurotoxicity.More importantly,the nano-materials are wrapped by nerve cell membrane,which improves the biocompatibility and brain targeted delivery of the materials,and promotes the expression of TH and the clearance of α-syn aggregates in MPTP mouse PD model.Conclusion:In this work,a BCFO@LCM drug loading platform that can respond to magnetic field was constructed,and it showed excellent effect in the established mouse Parkinson’s disease model,which significantly improved the behavioral defects of Parkinson mouse and increased the TH level in the brain tissue of Parkinson mouse.This is the first application of magnetoelectric nano-materials in Parkinson’s disease,and the successful application of the drug-loaded platform further shows that magnetoelectric nano-materials have broad application prospects in the field of tertiary prevention of neurodegenerative diseases.