Expression Significance And Correlation Of JMJD6 And VEGF In Cervical Cancer

PurposeCervical cancer is a global malignant tumor,and its incidence rate is the highest among female reproductive system malignant tumors.Therefore,we should search for the relevant molecules of the pathogenesis of cervical cancer,and provide new strategies for further clinical diagnosis and treatment.This paper will focus on the expression of JMJD6 and VEGF proteins related to cervical cancer.Immunohistochemical methods were used to detect the expression levels of JMJD6 and VEGF proteins in cervical squamous cell carcinoma tissue,cervical squamous intraepithelial lesion(SIL)tissue,and normal cervical tissue,and to analyze the correlation between the two.Combining clinical indicators,explore the correlation between the expression levels of JMJD6 and VEGF proteins and different clinical pathological features of cervical cancer,providing a new approach for early detection and diagnosis of cervical cancer.MethodsFirstly,we collected patients who underwent cervical squamous cell carcinoma surgery,SIL patients,and patients who underwent total hysterectomy due to benign uterine lesions(such as adenomyosis)from January 2000 to July 2020 in our hospital.Secondly,pathological tissue samples(paraffin sections)were grouped and obtained:squamous cell carcinoma group(20 cases of cervical squamous cell carcinoma patients),SIL group(20 cases of SIL patients),and control group(normal cervical tissue,taken from 20 patients who underwent total hysterectomy for benign uterine lesions(such as adenomyosis)at the same time).Finally,immunohistochemical techniques were used to detect the expression levels of JMJD6 and VEGF proteins in the three groups mentioned above,and statistical methods were used to investigate the correlation between the expression levels of JMJD6 and VEGF proteins and pathological parameters for cervical cancer diagnosis,such as age,FIGO stage,differentiation level,and the presence or absence of lymph node metastasis.Study the correlation between JMJD6 and VEGF in cervical cancer patients through Kappa analysis.Result1.Using immunohistochemical staining technology,JMJD6 was not expressed in normal cervical tissue,but showed low expression in cervical SIL tissue,with a positive rate of 15%(3/20).It was highly expressed in cervical squamous cell carcinoma tissue,with a positive rate of 80%(16/20);VEGF showed low expression in normal cervical and cervical SIL tissues,with positive rates of 10%(2/20)and 25%(5/20),respectively.Cervical SIL tissues were slightly higher than normal cervical tissues,while VEGF expression was significantly increased in cervical squamous cell carcinoma tissues,with a positive rate of 85%(17/20).2.Through Fisher’s exact probability test,there was a significant difference in the expression of JMJD6 and VEGF between the cervical squamous cell carcinoma group and the SIL group and the control group,P<0.05,with statistical significance.However,there was no significant difference in the expression of JMJD6 and VEGF between the SIL group and the control group,P>0.05.3.Statistical analysis using Fisher’s exact probability test showed that the expression rate of JMJD6 in cervical squamous cell carcinoma varies with age,FIGO stage,degree of differentiation,and the presence or absence of lymph node metastasis.The results showed that JMJD6 was closely related to the degree of differentiation of cervical squamous cell carcinoma(P=0.013,P<0.05),and the difference was statistically significant.There was no significant correlation between pathological parameters such as patient age,FIGO staging,and lymph node metastasis,and the difference was not statistically significant.4.The Kappa consistency test showed consistency in the expression of JMJD6 and VEGF proteins in cervical squamous cell carcinoma,with a Kappa value of0.828.Conclusion1.JMJD6 and VEGF are highly expressed in cervical squamous cell carcinoma tissue compared to normal cervical tissue and cervical SIL tissue,and the difference is statistically significant.However,both showed low expression in normal cervical tissue and cervical SIL tissue,and the difference was not statistically significant.2.The expression of JMJD6 is correlated with the degree of differentiation of cervical squamous cell carcinoma,P<0.05,with statistical significance.However,there was no correlation with age,FIGO staging,and lymph node metastasis,and there was no statistical significance.3.The expression of JMJD6 and VEGF protein is consistent in cervical squamous cell carcinoma.