Preparation Of Natural Tussah Silk Protein Nanofiber Membrane And Its Application In Repairing Bone Defect

Silk is a natural fibrous polymer.Silk protein,as the main component of silk,has application potential in regenerative medicine.Increasing application remains for silk materials in bone tissue engineering due to their characteristics in biocompatibility,biodegradability and mechanical properties.Plenty of the in vitro and in vivo studies confirmed silk is processed into scaffolds,hydrogels,and films to facilitate different bone regenerative applications.In this paper,based on the research at home and abroad,we summarize and analyze various methods of preparing silk protein nanofibers,and choose the physical fiber separation method to prepare natural silk nanofiber nonwoven membrane.In this paper,sodium carbonate was used to degum the silk,and then the degummed silk was physically separated by a wall breaker,and the silk fibroin nanofiber membrane was prepared.In this paper,it is found that micron natural silk fibroin fibers can be divided into nano and submicron natural silk fibroin fibers after degumming and physical analysis.Tussah silk nanofiber(TSn)and Bombyx mori silk nanofiber(BSn)membranes were prepared by physical shearing.The feasibility of using the two materials for guided bone regeneration was preliminarily investigated.Both membranes have excellent mechanical properties.The results showed that the two materials have the structural characteristics of nanofiber membrane,showing good biocompatibility,mechanical properties and the ability to guide bone regeneration.Meanwhile,compared to the BSn membrane,the TSn membrane has more excelle nt bionic structure and bone inductivity,making it an ideal candidate for guided bone regeneration.In the second part of the experiment,the amidation reaction between amino group and anhydride was further used to graft the carbon-carbon double bond to the silk protein macromolecular chain and the molecule of deferoxamine respectively.We hope to endow the fibrous membrane with vasogenic properties by loading it with deferoxamine.Through animal experiments,this study found that the group loaded with deferoxamine has better osteogenic characteristics,and the exact mechanism of its action needs to be explored in later experiments.