Effects Of NCAPG On The Proliferation,migration,and Invasion Of Ovarian Cancer Cells Via AKT Pathway And Its Mechanism

ObjectiveTo investigate the role of NCAPG in the proliferation,migration,and invasion of ovarian cancer cells and to describe the underlying mechanism.MethodsThe bioinformatics database was used to analyze the differential expression of NCAPG in ovarian cancer tissues and its association with the survival prognosis in ovarian cancer patients.Normal ovarian epithelial cells IOSE80,ovarian cancer cells A2780 and SKOV3 were cultured in vitro,and the protein expression of NCAPG in the three cells was measured by Western blot.NCAPG si RNA was transfected to construct knockdown NCAPG ovarian cancer cell models,and the experiments were divided into Blank group,Control group,si NCAPG-1 group,and si NCAPG-2 group.NCAPG overexpression plasmids were transfected to construct ovarian cancer cell models that overexpress NCAPG.The experiments were divided into Blank group,Control group,NCAPG group,NCAPG+MK2206 group,and MK2206 group(MK2206 is a specific inhibitor of the AKT signaling pathway).Western blot and q PCR to verify transfection efficiency,MTT assay to detect cell proliferation activity,cell scoring assay,and Transwell assay to analyze cell migration and invasion ability.Western blot to examine the expression levels of p-AKT,t-AKT,and downstream proteins PCNA,MMP-9,and EMT-related proteins in each group of cells.ResultsBioinformatics database analysis showed that NCAPG was overexpressed in ovarian cancer tissues and that patients with high NCAPG expression had a lower overall survival rate and poor prognosis.Western blot results showed that NCAPG expression was sequentially upregulated in normal ovarian epithelial cells IOSE80,ovarian cancer A2780,and SKOV3 cells.Compared with the Control group,the proliferative activity,scratch migration rate,and the number of invasive cells of ovarian cancer A2780 and SKOV3 cells were significantly reduced after silencing NCAPG;Western blot results showed that the expression of p-AKT,PCNA,MMP-9,EMT-related protein Vimentin,and N-cadherin protein were reduced and E-cadherin expression was increased.Compared with the Control group,overexpression of NCAPG significantly promoted the proliferation,invasion,and migration ability of ovarian cancer A2780 cells;Western blot results showed that p-AKT,PCNA,MMP-9,Vimentin,and N-cadherin protein expression was increased,and E-cadherin expression was decreased in the overexpression NCAPG group.AKT inhibitor(MK2206)significantly attenuated the above effects of NCAPG.ConclusionsNCAPG promote the proliferation,invasion,and migration of ovarian cancer cells by activating the AKT signaling pathway and regulating the expression of PCNA,MMP-9,and EMT-related proteins.