Application Of Surface Modified Polyetheretherketone Of Magnesium Chondroitin Sulfate In Bone Defects

1.Research Background:Bone defect is a very common orthopedic disease.With the popularization of transportation and the development of mechanized production,high-energy accidental injuries cause more and more limb trauma,and high-energy injuries caused by limb trauma is often easy to develop into different degrees of bone defects.In addition,environmental damage and pollution have led to a high incidence of bone tumors and various bone diseases,which is also the reason that there have been more and more cases of bone defects in recent years.The treatment of bone defects is varied,of which bone grafting is a proven and widely used technology.With the development of biomedical materials engineering,many bone replacement materials with biological and physical properties that are very close to normal bone tissue of the human body have been developed which providing more new ideas for the clinical treatment of bone defects.Polyetheretherketone(PEEK)is a polymer material with an elastic modulus very similar to human bone tissue,and it has good chemical resistance and biocompatibility,which is a very promising bioengineering material.However,the biological inertness of PEEK limits its application.2.Purpose and Significance:Based on the above research background,the surface modification of PEEK was carried out in this study through the sulfonation reaction method of concentrated sulfuric acid.Micropores were introduced on the surface of PEEK to improve its biocompatibility,improve its bioinerty and do not affect its mechanical properties.Dopamine(DPA)coating and chondroitin sulfate(MgCS)were introduced on the sulfonated PEEK surface by immersion method.Through surface modification of PEEK,it is hoped that MgCS-D-SPEEK prepared by chemical methods in this study can improve the biological inertility of PEEK,and improve angiogenesis,anti-inflammatory,osteogenesis induction and other effects.3.Research methods,content and process:3.1 Surface characteristics detection:The surface morphology,surface element distribution,hydrophilic ability and other characteristics of the prepared MgCS-D-SPEEK were evaluated by Search Engine Marketing(SEM),Energy Dispersive Spectroscopy(EDS),Water contact angle analysis,X-ray photoelectron spectroscopy(XPS)detection.3.2 Cell Experiment:By comparing the CCK-8 test and RT-qPCR test results of PEEK,D-SPEEK and MgCS-D-SPEEK in co-culture with MC-3T3 cells and HUVEC cells,the biocompatibility and safety of MgCS-D-SPEEK prepared in this study were verified.At the same time,whether it can improve osteogenic activity and angiogenesis activity,reduce inflammation and other effects.3.3 Animal experiment:Adult male SD rats were selected,tibia bone defects were constructed under anesthesia.And then PEEK,D-SPEEK and MgCS-D-SPEEK were implanted respectively.There was one control groups:sham operation group.The rats were divided into two groups according to the postoperative time of 1 month and 2 months.Micro-CT detection,HE staining and Goldner staining were performed to verify the osteogenic effect of rats.HE staining was performed on other important organs(including heart,liver,lung,spleen and kidney)of experimental rats to verify the biological safety of modified PEEK.4.Conclusions:MgCS-D-SPEEK prepared in this study has achieved the effect of improving PEEK’s biological inertness,and has the effects of improving angiogenesis,anti-inflammatory and inducing osteogenesis,etc.,which makes MgCS-D-SPEEK prepared in this study expected to become a new bone tissue engineering orthopedic implant.