The Study Of The Effects Of TRPM2 Gene Methylation And Childhood Trauma On Depressive Disorder

Objective:This study aims to investigate the relationship between TRPM2 gene and childhood trauma and depressive disorder by comparing the characteristics of TRPM2 gene methylation and childhood trauma in patients with depressive disorder with those in normal subjects,so as to explore the possibility that TRPM2 gene methylation combined with childhood trauma affects the occurrence of depressive disorder and its predictive value.Methods:A total sample of 80 patients with depressive disorder who were hospitalized in Shandong Mental Health Center from January to December 2022 and a control group of 80 healthy individuals from the health screening center during the same period were taken.Blood samples from 160 study subjects were tested for TRPM2 gene methylation sites and analyzed for differential methylation sites.The Childhood Trauma Questionnaire was used to assess the level of childhood trauma in members of both groups.The scale results and the degree of gene methylation were compared between the two groups.Logistic regression and receiver operating characteristic curves(ROC)were used to explore the effect of TRPM2 gene methylation level and childhood trauma level on the risk of depressive disorder and their predictive value for depressive disorder.Results:1.The depressive disorder group had significantly higher methylation level at the seven CpG sites of the TRPM2 gene(cg10095968、cg12300582、cg11818343、cg01380999、cg18560778、cg01368089、cg09765310)than healthy controls(P<0.0007),and the methylation level of all seven CpG sites could influence the risk of depressive disorder(OR=1.809～2.771,P<0.05).2.The total score of childhood trauma scale and the scores of emotional abuse,physical abuse,emotional neglect and physical neglect subscales were higher in the depressive disorder group than in the healthy control group(P<0.05),and all four childhood trauma subscale scores could influence the risk of depressive disorder(OR=1.147-1.372,P<0.05)3.Three CpG sites of the TRPM2 gene(cg01368089,cg18560778,cg12300582)were able to predict the onset of depressive disorder in combination with emotional abuse,with an area under the curve(AUC)of 0.812(95%CI:0.742～0.869,P<0.001)and the predictive value of TRPM2 gene methylation level together with emotional abuse was greater than its predictive value alone for depressive disorders(TRPM2 gene methylation level:AUC=0.755;emotional abuse:AUC=0.706).The difference was statistically significant.Conclusion:1.Elevated methylation of the TRPM2 gene is associated with an increased risk of depressive disorder.2.Childhood trauma increases the risk of depressive disorder.3.TRPM2 gene methylation combined with emotional abuse can predict the onset of depressive disorder.