The Tumor Suppressive Effect Of Heshu Xiaoji Prescription On H₂₂ Tumor-bearing Mice And The Effect Of Tumor VEGF And PDGF Proteins

Objective:To investigate the anti-tumor effect of Heshu Xiaoji prescription（HXP）on H₂₂tumor-bearing mice and the influence of tumor angiogenesis related factors on the expression of VEGF and PDGF proteins,exploring an effective treatment plan for hepatocellular carcinoma by combining traditional Chinese and Western medicine.Methods:H₂₂tumor-bearing mice were randomly divided into blank group,model group,low,medium and high dose groups of HXP（1.37,2.73,5.46 g/kg）,sorafenib group（0.03 g/kg）,combined group（5.46 g/kg HXP combined with sorafenib group 0.03 g/kg）,and the intervention was carried out for 14 days.After the experiment,tumor volume,relative proliferation rate and tumor suppression rate were calculated,AST and ALT liver function indexes were measured,tumor cell structure and morphology were observed by HE,and vascular endothelial growth factor（VEGF）and platelet-derived growth factor（PDGF）protein expression levels were measured by immunohistochemistry and Western blotting in the tumor tissues of each group.Results:There was no significant effect（P>0.05）on body weight change in mice,and HXP improved the mental and activity status and diarrhea symptoms caused by sorafenib in H₂₂tumor-bearing mice.Compared with the control group,all treatment groups inhibited the volume and mass of subcutaneous tumors in tumor-bearing mice,among which the relative tumor proliferation rate and tumor inhibition rate in the combined group were 31.3%and 74.8%,respectively,with the most obvious effect.HXP down-regulated AST and ALT expression levels and improved liver function in mice（P<0.05）.HE staining showed that the internal structure of tumor tissues was disturbed in each treatment group,which was positively correlated with the dose of HXP,and the most significant in the combined group（P<0.05）.IHC and Western blotting experiments revealed that the protein expression of VEGF and PDGF were decreased in each treatment group compared with the model group（P<0.001）;the combined group inhibited VEGF and PDGF more significantly than the single sorafenib or herbal medicine group.Conclusion:HXP can down-regulate tumorigenesis-related factors VEGF and PDGF protein expression,and has a tumor-suppressive effect on H₂₂bearing mice,which is most significantly treated with a combination of Chinese and Western medicine in combination with sorafenib.