The Study Of Early Predictive Value Of Three-dimensional Ultrasound For Fetal Growth Restriction And The Role Of EGLN3 On The Migration And Invasion Of Trophoblast Cells

Part 1:Early predictive value of three-dimensional ultrasound for fetal growth restrictionObjectives:To investigate the role of three-dimensional ultrasound in early prediction of fetal growth restriction.Methods:Ultrasounic placental parameters,such as the three-dimensional power doppler vascular index(Ⅵ),flow index(FI),vascular flow index(VFI),placental volume(PV)and placental quotient(PQ=placental volume/crown-rump length)were acquired by 3D ultrasound and virtual organ computer-aided analysis(VOCALTM)in the first trimester pregnancy.The intraclass correlation coefficient(ICC)between different operators was measured by randomly selecting 30 participants.Receiver operating characteristic(ROC)curve analysis evaluated the ability of each variable to predict FGR,and then the best cut-off point was found by using Youden index.Results:The intra-group correlation coefficient(ICC)of operators more than 0.80.With 457 pregnancies enrolled,35 delivered FGR(7.6%).The ultrasounic indicators of PV,PQ,VI,FI and VFI were markedly lower in the FGR pregnancies versus unaffected pregnancy.And the areas under the ROC curves(AUCs)of PV,PQ,VI,FI and VFI for FGR prediction were 0.751,0.772,0.870,0.652 and 0.873,respectively.Conclusions:The result suggests that VI,FI,VFI,PV and PQ in the first trimester pregnancies are predictors factors of FGR.And further findings revealed that the optimum parameter for prediction of FGR pregnancies are vascular index(VI)and vascular flow index(VFI)in the first trimester.In addition,the cutoff values may provide reference values for the early prediction of FGR.Part 2:The effections of EGLN3 on migration and invasion of trophoblast cellsObjectives:Bioinformatics analysis was used to screen out new genes that were significantly differentially expressed in fetal growth restriction.And we would explore the role of new genes in placental development of fetal growth restriction.Methods:We searched for the data sets with the conditions,which is human placental tissue with intrauterine growth restriction.And the original files of GSE100415 and GSE147776 data sets were downloaded from GEO database to screen out new genes that were significantly differentially expressed in fetal growth restriction.Western blotting and QRT-PCR were used to detect the expression of differential genes in fetal growth restriction placental tissues.After siRNA silencing EGLN3 gene,CCK-8 assay was used to verify the effect of EGLN3 on cell viability of HTR8/SVneo human villus trophoblast cells.The change of migration and invasion ability of human villus trophoblastic cells in HTR8/SVneo was detected by scratch assay and Transwell assay.Results:Bioinformatics analysis showed that EGLN3 was highly expressed in fetal growth restriction tissues and was related to placental oxidative stress.Western blotting and QRT-PCR results showed that EGLN3 was highly expressed in fetal growth restriction placental tissues.After the expression of EGLN3 gene was silenced in HTR8/SVneo human villus trophoblast cells,the proliferation ability of HTR8 cells was enhanced by CCK-8 assay.Scratch assay and Transwell assay showed that HTR8/SVneo human villus trophoblast cells had prmoted migration and invasion ability.Conclusions:The results of this study suggest that EGLN3 plays an important role in placental development with fetal growth restriction.Knockdown of EGLN3 gene expression can increase the proliferation and invasion ability of HTR8/SVneo human villous trophoblast cells,so EGLN3 may be a potential diagnostic marker for fetal growth restriction.