Correlation Analysis Of Gut Microbiota In Patients With Breast Cancer And Benign Breast Lesions

Objectives: Breast cancer and benign breast lesions are common diseases in women.The gut microflora may influence the development and treatment of breast diseases by participating in hormone metabolism and immune modulation.Hence,we explored gut microflora characteristics in patients with breast cancer and benign breast lesions.Methods: A cohort of 66 subjects was enrolled in the study.Of these,26 were breast cancer patients,20 were benign breast lesions patients,and 20 were matched healthy controls.The gut microbiota communities of cases were analyzed and compared with controls by sequencing the V3-V4 hypervariable regions of 16 Sr RNA gene.Results: Chao1,Ace,Shannon,Simpson indexes showed no differences among 3 groups(P>0.05).Both unweighted and weighted Uni Frac distances differed significantly among 3 groups(P<0.01).Peptoniphilus,Prevotella,Megamonas,Porphyromonas were potential biomarkers in breast cancer patients(LDA score>2).Escherichia,Lactobacillus and Coprobacillus were potential biomarkers in patients with benign breast lesions(LDA score>2).Eubacterium,Christensenella,Anaerofilum,and Faecalibacterium were decreased in the breast cancer group(P<0.05),Alistipes,Collinsella and Butyricimonas were decreased in both the breast cancer and benign breast lesions groups compared to the healthy control group(P<0.05).Conclusions: Patients with breast cancer and benign breast lesions undergo significant changes in intestinal microbiota.The flora with changed abundance may play an important role in the development of breast cancer and benign breast lesions.