Analysis Of The Levels Of UCP1 And UCP3 Of Skeletal Muscle And Subcutaneous Fat In Rats Under Chronic Hypothermia And Hypoxia

Objective:To observe the changes of UCP1 and UCP3 levels in skeletal muscle and subcutaneous fat and the changes of serum glucose and lipid metabolism-related indexes in rats under chronic hypothermic and hypoxic conditions,and to explore the relationship between UCP1 and UCP3 and glucose and lipid metabolism,as well as chronic hypothermia and hypoxia.Methods:70 healthy male rats were randomly divided into control groups（temperature 22℃,O₂concentration 21%）,hypoxia group（temperature 22℃,O₂concentration 10%）,and hypothermic groups（temperature 22℃,O₂concentration10%）.5℃,O₂concentration of 21%),low temperature combined with hypoxia group（temperature of 5℃,O₂concentration of 10%）.The rats in each group were reared under different environmental conditions until the 14th and 28th days,and the rats were sacrificed by anesthesia to measure their body weight and detect serum FBG,TC,TG,HDL-C,FFA and other indicators;the contents of UCP1 and UCP3 in skeletal muscle and subcutaneous fat of rats were detected by immunohistochemical technique.Result:1.Under the simple hypothermia or hypoxia environment,the changes of body weight and serum glucose and lipid metabolism indexes of rats were similar.The serum TC and TG levels first decreased and then increased with the prolongation of treatment time（p<0.05）.FBG,HDL-C and FFA showed a trend of increasing first and then decreasing with time（p<0.05）;the expression levels of UCP1 and UCP3 in skeletal muscle and subcutaneous adipose tissue of rats increased first and then decreased gradually with time（p<0.05）.2.When the hypothermia and hypoxia environment was combined,the body weight and serum TC and TG levels of rats showed a trend of first decreasing and then increasing with the extension of hypothermic and hypoxia time（p<0.05）,while FBG,HDL-C and FFA showed increased first and then decreased with the extension of time（p<0.05）.The expression levels of UCP1 and UCP3 in the skeletal muscle and subcutaneous adipose tissue of rats increased first and then decreased with the extension of hypothermia and hypoxia time（p<0.05）.and the degree of change was more significant than that of simple hypothermia or simple hypoxia.3.Comparison between groups under different environmental conditions at the same time:（1）At 14 days,the comparison of the indicators in the control group,hypothermia group,hypoxia group and hypothermia combined with hypoxia conditions:body weight decreased most significantly in the hypothermia combined with hypoxia 14 days group（p<0.05）.serum TC and TG in the hypothermia 14 days group decreased most significantly（p<0.05）.while the serum levels of FBG,HDL-C,FFA,and the expressions of UCP1 and UCP3 in skeletal muscle and subcutaneous fat were significantly increased in the hypothermia combined with hypoxia for 14days（p<0.05）.（2）At 28 days,the comparison of the indicators in the control group,hypothermia group,hypoxia group and hypothermia combined with hypoxia conditions:The level of FBG in the hypoxia 28-day group was significantly higher than that in the other three groups（p<0.05）.The level of FFA in the hypothermia combined with hypoxia for 28 days was significantly taller than that in the other three groups（p<0.05）.there was no statistically significant difference in body weight,serum TC,TG and HDL-C levels,and UCP1 and UCP3 expression in skeletal muscle and subcutaneous fat among the four groups.（p>0.05）.Conclusion:hypothermia and hypoxia can induce the increased expression of UCP1 and UCP3 in skeletal muscle and subcutaneous adipose tissue,and metabolic changes occur at the same time,which are manifested as weight loss,blood lipid level,and fasting blood glucose increase;and when hypothermia is combined with hypoxia,the above indicators change more significantly.However,with the prolongation of time,the above indicators gradually tended to the level of the control group,and the body gradually became accustomed to the chronic hypothermic and hypoxia environment.