Screening And Identification Mouse TREM-1 Interaction Protein

Sepsis is a complex clinical symptom.Infectious and some non-infectious factors can lead to sepsis.At present,it is considered that excessive inflammation and organ damage are the main mechanism of sepsis.Myeloid cells trigger receptor 1(TREM-1)plays an important role in sepsis.On the one hand TREM-1 signaling pathways activated can induce immune cells to produce a series of inflammatory reaction,On the other hand TREM-1 signaling pathway can also with pattern recognition receptors TLRs and NODs to further expand the inflammatory response.But so far the TREM-1 ligand is still unknown.So the comprehensive elaboration the function of TREM-1 signaling pathways in sepsis is difficult,thus it greatly limits the research of sepsis.Some studies have shown that TREM-1 identify ligands existed on platelets.Given the important role of platelet in sepsis,our study intends to screening and identification protein in the platelet interact with TREM-1.Our study screenings 14 kinds of protein in the platelet by yeast two hybrid technology and Pull down technology,but only Actin interacts with TREM-1 through the double label pull down technology.Immunofluorescence confocal test further determined the interaction between TREM-1 and Actin,namely TREM-1 and Actin were co-location on the immune cell surface in the process of inflammation.At the same time,TREM-1 and Actin interaction could enhance the level of ROS produced by RAW264.7macrophages which activated by TREM-1 signaling pathways.In conclusion,our study identified Actin in the platelet interacts with TREM-1,and their interaction enhance the inflammatory response of immune cells.That proves Actin is the ligand of TREM-1.So our study provides important basis for study TREM-1 signaling pathway mediated inflammatory response mechanism.