The Role Of MicroRNA-203,microRNA-27b And Their Related Proteins In The Pathogenesis Of Cutaneous Lichen Planus

Objective:Lichen Planus(LP)is a chronic inflammatory and immune disease.Its exact etiology and pathogenesis remain unclear.Some scholars believe that its pathogenesis is related to micro RNA(miRNA).Micro RNA-203(miR-203)and micro RNA-27b(miR-27b)play important roles in regulating skin inflammation,keratinocyte proliferation and immune system differentiation.The expression of protein-coding genes can be dynamically regulated by targeted m RNA degradation or translation.However,Whether miR-203 targets interleukin-22(IL-22),interleukin-8(IL-8),miR-27 b and interleukin-1β(IL-1β)and matrix metalloproteinase-2(MMP-2)play a role in the pathogenesis of cutaneous lichen planus(CLP)is not clear.To this end,we carried out the following studies: 1.To detect the expression of micro RNA-203(miR-203)and its downstream target proteins interleukin-22(IL-22)and interleukin-8(IL-8)in lichen planus(LP)skin lesions and normal skin tissues.2.To detect the expression of micro RNA-27b(miR-27b)and its related proteins interleukin-1β(IL-1β)and matrix metalloproteinase-2(MMP-2)in LP skin lesions and normal skin tissues.3.To analyze the correlation between miR-203,miR-27 b and their target proteins and explore their biological significance in the pathogenesis of cutaneous lichen planus(CLP).Methods:1.Real-time PCR(RT-PCR)was used to detect the expression of miR-203 and miR-27 b in 40 LP skin lesions(LP group)and 40 normal skin tissues(control group).2.Enzyme-linked immunosorbent assay(ELISA)was used to detect the expression of IL-22,IL-8,IL-1β and MMP-2 in the two groups.3.Spearman was used to analyze the correlation between the expression of miR-203,IL-22 and IL-8,and between the expression of miR-27 b,IL-1β and MMP-2.Results:1.The expression of miR-203 and miR-27 b in the LP group were significantly down-regulated compared to the control(p<0.05).2.The expression of IL-22,IL-8,IL-1β and MMP-2 were significantly up-regulated compared to the control(p<0.05).3.There was a negative correlation between expression of miR-203 and IL-22,IL-8,and the latter two was positive(p<0.05).There was a negative correlation between miR-27 b and IL-1β,MMP-2,and the latter two was positive(p<0.05).Conclusion:MiR-203 and miR-27 b may negatively regulate the expression of downstream target proteins IL-22,IL-8,IL-1β and related protein MMP-2.Therefore,the decreased expression levels of miR-203 and miR-27 b may promote the occurrence of CLP.These results provide a useful reference for revealing the pathogenesis of LP and provide a theoretical basis for screening therapeutic targets in the future.