Study On The Effect And Application Of Mogrol On Lung Cancer

Objective: To investigate the antitumor effect and preliminary mechanism of Mogrol on lung cancer cells in vivo and in vitro.Methods: In this study,Lung cancer cells A549 and H1299 were treated with 0,25,50 and 100 μmol/L of different concentrations of Mogrol solution.MTT experiment,plate cloning experiment,scratch experiment,Transwell experiment and Hochest experiment were performed 33258 experiment and FITC/PI double staining assay for cell apoptosis were used to detect the effects of Mogorl on lung cancer cell proliferation,cell invasion and cell apoptosis,so as to evaluate the anti-tumor effect of Mogrol.Transcriptome sequencing technology was used to analyze the differentially expressed genes and explore the potential mechanism of Mogrol.Ten differentially expressed genes in transcriptome were verified by QPCR assay,and proteins related to cycle,invasion,apoptosis and AKT/mTOR signaling pathway were verified by Western blot assay.The xenograft tumor model of lung cancer cell A549 was established to evaluate the in vivo therapeutic effect of Mogrol on lung cancer.Results: In vitro experiments,Mogrol had significant anti-tumor effect,inhibited the proliferation,invasion and migration of lung cancer cells and induced the apoptosis of lung cancer cells.Transcriptome sequencing results showed that 3563 differentially expressed genes were found in lung cancer cells treated with Mogrol and were closely related to AKT/mTOR signaling pathway.QPCR experiment verified that 10 differentially expressed genes were consistent with the results predicted by transcriptome sequencing technology.Western blot results showed that the antitumor effect of Mogrol was induced by inhibiting AKT/mTOR signaling pathway.Mogrol could also regulated the expression of cycle,apoptosis and invasion-related proteins to inhibit the occurrence and development of lung cancer cells.In vivo experiments,the tumor-bearing mice treated with Mogrol showed significant therapeutic effects compared with the control group,and the mechanism of therapeutic effects was related to the regulation of invasion,apoptosis,cyclin and inhibition of AKT/mTOR signaling pathway.Conclusions:Our results indicated that Mogrol could effectively inhibited the occurrence and development of lung cancer cells,and the anti-tumor effects are exerted mainly through inhibition of cell proliferation,invasion and induction of apoptosis.These processes were mediated by the AKT/mTOR signaling pathway.Mogrol may be a potential drug as a molecular targeting strategy for cancer therapy.