| Obj ectiveTo observe the effects of Jiaji electro-acupuncture combined with treadmill training on the microstructure of tibia,the spinal cord of T12-L1 segment,the expressions of NGF and TrkA in the sciatic nerve and dorsal root ganglion on the injured side,and the expression of NGF mRNA and protein in the tibia of rats after scatic nerve injury.As well as to explore the effect of Jiaji electroacupuncture combined with treadmill training on the improvment of bone quality in rats after peripheral nerve injury and the related regulatory mechanism.MethodsUsing random number table method,180 SD male rats were divided into 5 groups,36 rats in each group:normal control group,sciatic nerve transection control group(SNTC),Jiaji el ectroacupuncture group(EA),treadmill group,Jiaji electroacupuncture combined with treadmill group(ET).The rats in each group were divided into 3 subgroups of 12 rats in each subgroup according to the time points of 2 weeks,4 weeks and 8 weeks after the treatment.Except for the normal control group without any intervention,the rest of the groups were modeled according to the sciatic nerve cutoff and suture model.After the model was built,the rats in the SNTC group were put into the cage,and no other treatment was performed except for food and water,and the bedding was changed regularly.;the EA group was treated with Jiaji electroacupuncture at the cor-responding segment;the rats in the treadmill group were given regular treadmill-related training;the ET group was given Jiaji electro-acupuncture as well as treadmill training.At each time point,Micro-CT was used to analyze the changes of rat tibia microstructure,RT-PCR and Western blot techniques were used to detect the of NGF mRNA and protein expression in rat tibia,and immunohi stochemi stry was used to detect the NGF and TrkA expression in the sciatic nerve,T12-L1 dorsal root ganglia and spinal cord.Results1.The results of Micro-CT detection of tibia microstructure of rats in each group:at 2 weeks,4 weeks and 8 weeks after treatment,the relative bone volume fraction(BV/TV),the number of trabecular bone(Tb.N)and trabecular bone thickness(Tb.Th)were higher than those in the SNTC group,and lower than those in the normal control group(P<0.05),while the trabecular bone separation(Tb.Sp)were lower than those in the SNTC group,and higher than the normal control group(P<0.05);from the horizontal comparison of treatment time points,the parameters of tibial microstructure were best at 8 weeks;the results of the ET group were better than the EA and treadmill group alone at each time point.2.The expression of NGF mRNA and protein in tibial bone tissue of rats in each group:at 2 weeks,4 weeks and 8 weeks after treatment,except for the normal control group,RT-PCR detected that the NGF mRNA in the tibia of rats in each group showed a trend of first rising and then decreasing,and reached the peak at the time point of 2 weeks;at each time point,the NGF mRNA of the ET group was significantly higher than that of the normal control group,SNTC group,the EA group and the treadmill group(P<0.05);at each time point,except for the normal control group,the expression of NGF protein in the tibia tissue of rats in western blot detection also showed a trend of first rising and then decreasing,peaking at 4 weeks and then decreasing;at 2 weeks and 4 weeks after treatment,the expression of NGF protein in the tibial tissue of rats in the ET group was significantly higher than that of the SNTC group(P<0.05);at 8 weeks after treatment,the expression of NGF protein in the tibia tissue of rats in the ET group was significantly higher than that of the SNTC group and the EA or treadmill group alone(P<0.05).3.The NGF expression results of rats in each group:at 2 weeks,4 weeks and 8 weeks after treatment,compared with the normal control group,the NGF expression in the segment spinal cord,the injured side sciatic nerve,and the dorsal root ganglia were decreased,showing a decline and then an increase,reaching a peak at 4 weeks.①Segmental spinal cord:After 2 weeks of treatment,NGF expression in the ET group was significantly higher than that in the SNTC group(P<0.05)and lower than that in the normal control group;at 4 weeks and 8 weeks after treatment,the NGF expression in the ET group was significantly higher than that in the normal control group,SNTC group and EA or treadmill group alone(P<0.05);②Sciatic nerve:at each time point,the NGF expression of each intervention group was significantly higher than that of the SNTC group(P<0.05)and lower than that in the normal control group;at 4 weeks and 8 weeks after treatment,the NGF expression of the ET group was significantly higher than that of the normal control group and EA group and the treadmill group alone(P<0.05);③Dorsal root ganglia:at each time point,the NGF expression in the ET group was significantly higher than that in the SNTC group(P<0.05);at 4 weeks and 8 weeks after treatment,the NGF expression in the ET group was significantly higher than that in the normal control group,EA group and the treadmill group alone(P<0.05).4.The TrkA expression results of rats in each group:At 2 weeks,4 weeks and 8 weeks after treatment,compared with the normal control group,the TrkA expression in the segment spinal cord,the injured side sciatic nerve and the dorsal root ganglia of the model control group were decreased,showing a trend of first decreasing and then rising and then declining,reaching a peak at 4 weeks.①Segmental spinal cord:At 2 weeks after treatment,the TrkA expression in the ET group was significantly higher than that in the normal control group and SNTC group(P<0.05);at 4 weeks and 8 weeks after treatment,the expression of TrkA in the ET group was significantly higher than that in the normal control group,SNTC group and the EA group or treadmill group alone(P<0.0 5);② Sciatic nerve:at each time point,the expression of TrkA in each intervention group was significantly higher than that in the SNTC group(P<0.05);At 2 weeks after treatment,the expression of TrkA in the sciatic nerve in the injured side of the ET group was higher than that of the normal control group,treadmill training group and Jiaj i electroacupuncture group;At 4 and 8 weeks after treatment,the expression of TrkA in the sciatic nerve on the injured side of the ET group was significantly higher than that of the normal control group,Jiaji EA group and treadmill group(P<0.05);③Dorsal root ganglia:at each time point,the expression of TrkA in the ET group was significantly higher than that in the normal control group and the SNTC group(P<0.05);at 4 weeks and 8 weeks after treatment,the expression of TrkA in the ET group was significantly higher than that in the EA group or the treadmill group alone(P<0.05).Conclusions1.The application of Jiaji electroacupuncture combined with treadmill training can improve the microstructure of the tibia after sciatic nerve injury,as well as promote the expression of NGF and TrkA protein in the segmental spinal cord,the injured lateral sciatic nerve and the dorsal root ganglia,as well as the expression of NGF mRNA and protein in the tibia.And the effect of Jiaji electroacupuncture combined with treadmill training is better than that of Jiaji electroacupuncture or treadmill training alone.2.The improvement of tibial microstructure by Jiaji electroacupuncture combined with treadmill training may be related to the up regulation of NGF mRNA and protein expression and the promotion of TrkA expression. |
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