Clinical Significance Of Lymphocyte Subsets In Iron Overload Patients With Thalassemia

Objective: To study the effect of iron overload on peripheral blood lymphocyte subsets in patients with thalassemia and its clinical significance,try to analyze the causes of susceptibility to thalassemia infection,and provide theoretical basis for the prevention and treatment of thalassemia infection.Methods: A total of 58 confirmed cases of thalassemia were selected and divided into 2groups according to the concentration of serum ferritin(SF).Serum ferritin concentration >1000ug/L was the iron overload group,with a total of 31 cases;the concentration of serum ferritin <1000ug/L There were 27 cases of non-iron overload.At the same time,30 healthy people from the physical examination center of our hospital during the same period were selected as the control group.Flow cytometry was used to detect the level of lymphocyte subsets,and the blood cell classification and count,ferritin,iron levels and clinical data were collected.To analyze the expression levels of iron-overloaded lymphocyte subsets in thalassemia patients,and to explore the influence and clinical significance of iron overload and lymphocyte subsets in thalassemia patients.Results:(1)There were significant differences in the number of lymphocytes and monocytes among the three groups(all P<0.05).The number of lymphocytes in the iron overload group was lower than that in the non-iron overload group.Compared with the iron overload group,the difference was statistically significant(P<0.05).There was no significant difference in the multiple comparison of neutrophils among the three groups(P>0.05).The difference in cells was statistically significant(all P<0.05).The proportion of CD4+～T cells and NK cells in iron overload thalassemia patients was lower than that in the control group;while CD3+～T cells,CD4+～T cells/CD8+～T cells,there was no significant difference in B cells and CD8+～T cells among the three groups(all P>0.05).(3).There was a negative correlation between CD4+～T cells and ferritin in iron overload group(r=-0.52,P<0.05).There was a negative correlation between NK cells and ferritin in iron overload group(r=-0.55,P<0.05),both of which were moderately negatively correlated;CD4+～T cell NK cells in non-iron overload group were not correlated with serum ferritin(all P >0.05)Conclusion: The distribution characteristics of lymphocyte subsets in patients with iron overload thalassemia are different from those in non-iron overload thalassemia patients.High iron load may affect immune function in patients with thalassemia.