Protective Effect Of Ginsenoside Rg1 On Renal Fibrosis In Diabetic Nephropathy

The incidence of diabetic kidney disease(DKD)is increasing year by year,and now it has become the most hospitalized chronic kidney disease(CKD)in China,and DKD is progressing rapidly.With the progress of the disease,DKD patients gradually appear glomerulosclerosis,tubulointerstitial fibrosis and other pathological changes,which leads to the gradual loss of normal function of the kidney and the entry into end-stage renal disease(ESRD).At present,the treatment of DKD is limited,mainly to control blood sugar,blood pressure,lipid regulation and other treatment,but the current treatment can not completely block the progress of DKD,it is urgent to find new treatment and drugs.Ginsenoside Rg1,an extract of Panax ginseng,has been found to have protective effects on many diseases through anti-inflammatory and antioxidant stress,while oxidative stress and inflammation play an important role in the fibrosis progression of DKD.However,there are few studies on the intervention effect of ginsenoside Rg1 on DKD.Objective: To explore the protective effect and mechanism of ginsenoside Rg1 on DKD renal fibrosis.Methods:1.Prediction of the Protective effect of ginsenoside Rg1 on Diabetic Nephropathy by Network Pharmacology;2.The animal model of diabetic nephropathy was established in C57BL/6J strain mice by high fat and high sugar diet combined with streptozotocin,and the intervention effect of ginsenoside Rg1 on DKD was verified in vivo;3.DKD podocyte injury model was established by inducing MPC-5 renal podocytes induced by high glucose.Nrk-52 E renal tubular epithelial cells were induced by TGF-β1 to construct fibrotic tubular cell model.The intervention effect of ginsenoside Rg1 on the cell model was observed.Results:1.Through the prediction of network pharmacology,it is found that ginsenoside Rg1 can protect DKD patients through anti-inflammation and antioxidation,and delay the fibrosis progression of DKD by interfering with TGF-β-mediated downstream signal pathway.2.Through animal experiments,it was found that diabetic animal mode co uld be established in C57BL/6J mice by high-fat and high-sugar diet combined with streptozotocin,but it was difficult to construct DKD animal model.Due to the long construction time and many influencing factors of DKD animal model,the in vivo verification of ginsenoside Rg1 could not be carried out.3.Through cell experiment,it was found that ginsenoside Rg1 could inhibit TGF-β1/Smad3 signal pathway activated by MPC-5 podocytes in high glucose environment.It also decreased the expression of Smad3 and Fibronectin(FN)in Nrk-52 E renal tubular epithelial cells induced by TGF-β 1,thus delaying the occurrence of cell fibrosis.Conclusion: Ginsenoside Rg1 can delay the progression of renal fibrosis in DKD by interfering with TGF-β1-mediated signal pathway.