Expression And Clinical Significance Of ACSL1 In Hepatocellular Carcinoma

Objective:(1)To explore the expression leval and clinical significance of Long-Chain Acyl-Co A Synthetase 1(ACSL1)in hepatocellular carcinoma.(2)To explore the effects of ACSL1 on the proliferation and apoptosis of hepatocellular carcinoma.(3)To preliminarily explore the effects of ACSL1 on energy metabolism reprogramming in hepatocellular carcinoma.Methods:(1)Analyze expression level and clinical significance of ACSL1 in hepatocellular carcinoma,334 paired hepatocellular carcinoma tissues diagnosed by histological examination are collected to analyze the expression level of ACSL1 by immunochemistry staining.the paper also analyzes the relationship between the expression of ACSL1protein and the clinicopathological characteristics and prognosis of hepatocellular carcinoma patients by SPSS software.(2)ACSL1 effects on proliferation and apoptosis of hepatocellular carcinoma,Lentivirus transfection is used to overexpression ACSL1 in hepatocellular carcinoma cell line SNU739,and the results of lentivirus transfection are verified by Western blot assay.MTS and Flow cytometry are used to detect the effects of ACSL1 on the proliferation and apoptosis of SNU739 cells.(3)To explore the preliminary mechanism of ACSL1 in hepatocellular carcinoma,Cell energy metabolism reprogramming is detected by Seahorse XF24 analyer and Lactate Assay Kit.Results:(1)Clinical data analysis of hepatocellular carcinoma shows that the expression of ACSL1 in caner tissues is markedly lower than that in adjacent tissues(t=20.040,P<0.001)and the expression of ACSL1 is related to age of the patient(χ~2=16.472,P<0.001).the level of ACSL1 expression is a risk factor for survival in hepatocellular carcinoma patient(HR=1.642,P<0.01).(2)By analyzing the effect of ACSL1 on the proliferation and apoptosis of SNU739 cells,the results shows that compared with the control group,ACSL1 is significantly promoting cell apoptosis(t=3.886,P<0.001)and inhibited cell proliferation(F=45.056,P<0.01).(3)By measuring the effects of ACSL1 on the oxygen consumption rate and lactic acid content of SNU739 cells,the results shows that compared with the control group,ACSL1 significantly increases mitochondrial basic oxygen consumption rate(F=83.429,P<0.01)and maximal oxygen consumption rate(F=859.792,P<0.001)in SNU739 cell,While the lactic acid production inhibited(t=4.585,P<0.05).Conclusions:(1)Comparing with the corresponding adjacent tissues,The level of expression of ACSL1 is significantly lower in clinical liver cancer tissues.(2)The level of expression of ACSL1 of hepatocellular carcinoma patient is correlating with the age of patients.(3)The level of expression of ACSL1 is a risk factor for survival of hepatocellular carcinoma patients.(4)Comparing with the control group,ACSL1 can significantly inhibit the proliferation and promote the apoptosis of SNU739 cells.(5)Comparing with the control group,ACSL1 can significantly increase the mitochondrial oxygen consumption rate of SNU739 cells and reduce the production of lactic acid.