Inhibitory Effect Of Phloretin On Lps Induced Inflammation Of RAW264.7 Cells And Its Mechanism

Objective:To study the relationship between inflammatory cell model and root skin hormone induced by lipopolysaccharide.Finally,the effect of root skin on RAW264.7 macrophages was analyzed by microscopic inflammatory reaction data.The anti-inflammatory mechanism of root skin hormone was analyzed from different angles,which provided the corresponding scientific basis for clinical treatment of various diseases caused by inflammation.Methods:First of all,we need to determine the maximum dose of phloretin drug toxicity,so as to select the appropriate dose for the experiment.The toxicity of phloretin was analyzed by CCK-8 method.In order to better reflect the role of phloretin on inflammatory response,we used ELISA,real-time fluorescence quantitative PCR,Western blot,immunofluorescence(icc-if)methods to analyze the expression of inflammatory factors in cells.It provides the basis for clinical development of new anti-inflammatory drugs.Results:Through CCK-8 method,we found that when the concentration of phloretin was 1 μg/mL-80 μg/mL,there was no significant difference in cell proliferation(P<0.05).When the concentration of phloretin was 100 μg/ml,the cytotoxicity began to appear,and we can see from the figure that its toxicity increased significantly;through ELISA,real-time fluorescence quantitative PCR,Western blot analysis We found that there were significant differences in the results of different concentrations of phloretin intervention by using the methods of blot and immunofluorescence(icc-if).Compared with the normal group,the model group induced the high expression of related inflammatory factors and related inflammatory signaling pathways(P<0.05);compared with the model group,the drug groups with different concentrations of phloretin reduced the related inflammatory factors and related inflammatory signaling pathways The expression of signal transduction pathway(P<0.05).Conclusion:Phloretin could significantly inhibit the release of inflammatory factors,the mRNA expressions of TNF-α,IL-6,IL-1β,iNOS and COX-2,as well as the protein expressions of Akt/NF-κB,ERK1/2 and P38 in RAW264.7 cells induced by LPS.