Expression Of FGFRL1 In Colorectal Cancer And Its Overexpression On Biological Behavior Of Colorectal Cancer Cells

Objective:To investigate the expression of FGFRL1 in colorectal cancer and its effect on the biological behavior of HCT116 cells.Methods:The expression of FGFRL1 was detected by immunohistochemistry,and the relationship between the expression of FGFRL1 protein and the clinicopathological features was discussed by combining with the clinical data.FGFRLI cDNA was cloned into pcDNA3.1(+)plasmid,HCT116 cells were transfected with the recombinant plasmid and the stably expressed cell strains were constructed.In the following experiment,cells were divided into three groups:the blank group(untreated HCT116 cells),negative group(pcDNA3.1(+)transfected cells)and experimental group(recombinant plasmid transfected cells).q-PCR and Western blot were used to comfirm the overexpression of FGFRLI.The cell proliferation was detected by using CCK-8 kit.The cell migration and invasion ability were analysed by transwell chamber test.The cell apoptosis rate and cell cycle changes were evaluated by flow cytometry.Results:Immunohistochemistry showed that the expression of FGFRL1 protein in the adjacent normal tissues was higher than that in the cancer tissues.The analysis of clinical cases showed that the expression of FGFRL1 protein was only related to the degree of differentiation of colorectal cancer,but not to gender,depth of invasion,clinical stage,distant metastasis and lymph node metastasis.The results of q-PCR showed that the expression of FGFRL1 in the experimental group was 47.6168 times higher than that in the blank group.The results of western blot confirmed the overexpression of FGFRL1 in experimental group.The proliferation of HCT116 cells was significantly inhibited after overexpression of FGFRL1(P<0.05).Transwell chamber showed that overexpression of FGFRL1 significantly inhibited the migration of HCT116 cells(P<0.001).Flow cytometry showed that overexpression of FGFRL1 promoted HCT116 cell apoptosis(P<0.05)..Conclusion:FGFRL1 is down-regulated in colorectal cancer.Overexpression of FGFRL1 can inhibit the proliferation and migration of colorectal cancer cells,and promote cancer cell apoptosis.