Screening Of Biomarkers For Polycystic Ovary Syndrome Based On Metabolomics

Polycystic ovary syndrome(PCOS)is an endocrine disorder prevalent in women of childbearing age.It has a high degree of heterogeneity,with obesity,clinical or biochemical manifestations of hyperandrogenemia and abnormal glucose and lipid metabolism.Therefore,the exploration of the complex pathogenesis of PCOS,as well as the root causes of different physiological manifestations and metabolomic characteristics,which have guiding significance for the clinical selection of precise medical intervention measures.ObjectiveNon-target metabolomics study was conducted on the serum and urine between PCOS and normal people to identify the small molecule metabolites associated with the development of PCOS.The biomarkers with obvious correlation of PCOS were studied from the perspective of metabolomics,to analyze the pathogenesis of insulin resistance,clinical or biochemical manifestations of hyperandrogenemia and obesity in PCOS patients,which could provide theoretical basis for the treatment of PCOS patients with different characteristics.MethodsThe difference metabolites of serum and urine between PCOS group and normal group was analyzed by UPLC/Q-TOF-MS.According to the clinical and biochemical characteristics of PCOS patients such as insulin resistance,clinical or biochemical manifestations of hyperandrogenemia and obesity,to analyze the differences of metabolites in the groups.The original data were analyzed and processed by professional metabolomics software,and multivariate statistical analysis and univariate statistical analysis were performed.The model graph was constructed to screen the metabolic markers of PCOS patients according to VIP,Max FC value and P value,also the differences of metabolites in the subtype group of PCOS.ResultsCompared to PCOS group and normal group,35 serum metabolites with significant differences were identified.Specifically,11 metabolites were significantly increased and 24 metabolites were significantly decreased in PCOS group.The increased metabolites in PCOS group were as follows,5a-Dihydrotestosterone sulfate,PI(O-20:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z)),Ganglioside GM3,L-2-Amino-3Methylenehexanoic acid,PE(12:0/0:0),TG(8:0/8:0/8:0)etc.The decreased metabolites in PCOS group were as follows,1-Lyso-2-arachidonoyl-phosphatidate,Phenylalanyl phenylalanine,GangliosideGA2,Glucosylceramide(d18:1/26:1(17Z)),Palmitoleoyl Ethanolamide.Different metabolites in serum were stratified in PCOS group.There were 7 different metabolites in IR group and NIR group.There were 5 different metabolites in hyperandrogenemia group and non-hyperandrogenemia group.There were 6 different metabolites in obesity group and nonobesity group.Compared to PCOS group and normal group,10 urine metabolites with significant differences were identified.all metabolites were significantly decreased in PCOS groups.Specifically,Ascorbic acid,1-Stearoylglycerophosphocholine,3-Nitrotyrosine,Adenosine phosphosulfate,Prenyl glucoside etc.Differential metabolites in urine were stratified in PCOS group There is only one kind of urine differential metabolite,which is 1-Stearoylglycerophosphocholine.ConclusionThe changes of PCOS metabolites mainly involve amino acids,lipids,nucleosides,vitamins and organic acids,which are related to IR,obesity,hyperandrogenemia,infertility and abortion.Each subtype of PCOS has its unique metabolomic characteristics.There are degradation products of serum metabolites in urine.