Study On Application Of The Mixture Of Glycerol Monolaurate And Cinnamaldehyde In Laying Hens

The purpose of this experiment was to investigate the effects of dietary supplementation with different levels of 80%glycerol monolaurate and 20%cinnamaldehyde complex（GCM）on production performance,antioxidant function,and intestinal health in laying hens.Using a single factor experimental design,1120 healthy15-week-old Jing Fen No.1 laying hens were randomly divided into 4 treatments,each with 10 replicates and 28 chickens per replicate.Each treatment group was divided into a control group（without GCM added to the base diet）,a 250 mg/kg GCM group（with 250mg/kg GCM added to the base diet）,a 500 mg/kg GCM group（with 500 mg/kg GCM added to the base diet）,and a 1000 mg/kg GCM group（with 1000 mg/kg GCM added to the base diet）,with a pre feeding period of two weeks and a trial period of 12 weeks,The test results were as follows:1.Effects of GCM on laying performance,egg quality and serum biochemical indexes of laying hensCompared to the control group,all GCM addition groups could significantly reduce the unqualified egg rate of 16～20-week-old laying hens（P<0.001）.However,it had no significant effect on the average daily feed intake,feed egg ratio,egg production rate,average egg weight,egg production and unqualified egg rate of 21～28 weeks old laying hens（P>0.05）.In addition,the addition of 250 mg/kg and 1000 mg/kg GCM to the diet significantly increased the yolk color（P<0.05）of 20-week-old laying hens,but had no effect on the eggshell thickness.The addition of 250 mg/kg and 500 mg/kg GCM to the diet significantly increased the yolk color（P<0.05）of 24-week-old laying hens,and the addition of three levels of GCM significantly increased the Hastelloy unit（P<0.05）of eggs.Compared to the control group,the addition of GCM to the diet had no significant effect on the blood biochemical indexes of 20-and 28-week-old laying hens.In addition,it had no significant effect on the blood,ALB,TC,CA,LDL,UA and DB contents of 24-week-old laying hens,as well as the activity of ALT,ALP,GGT,CK and LDH in each group（P>0.05）,but the addition of 250 and 1000 mg/kg GCM in the diet increased the content of serum GLU（P<0.05）,and the addition of 250 and 500 mg/kg GCM could upregulate the content of serum HDL（P<0.05）.2.Effects of GCM on organ index,follicle count and antioxidant function of laying hensCompared to the control group,the addition of GCM had no effect on the liver,spleen and abdominal fat index of 28-week-old laying hens（P>0.05）.The addition of GCM of500 mg/kg and 1000 mg/kg significantly increased the number of small follicles（2 mm<follicle diameter<5 mm）（P<0.05）,while the addition of 1000 mg/kg GCM reduced large follicles（follicular diameter>）10 mm)（P<0.05）.The addition of 250 mg/kg GCM upregulated the serum T-SOD activity of 20-week-old laying hens and down-adjusted the content of CAT（P<0.05）.The addition of 500,1000 mg/kg GCM downregulated the content of MDA and H₂O₂in serum（P<0.05）.Compared to the control group,the addition of 1000 mg/kg GCM increased the viability of T-SOD and GSH-Px in the serum of 24-week-old laying hens（P<0.05）,the addition of three levels of GCM decreased the content of serum MDA（P<0.05）,and the addition of 250 and 500 mg/kg GCM downregulated the serum H₂O₂content（P<0.05）.The content of T-SOD in the liver of 28-week-old laying hens was adjusted by adding 250and 500 mg/kg GCM to the diet,and the content of H₂O₂（P<0.05）was adjusted by adding1000 mg/kg GCM.At the same time,T-SOD in the jejunum of the 500 mg/kg GCM group was significantly increased（P<0.05）.The addition of three levels of GCM upregulated jejunal GSH-Px activity（P<0.05）,and the content of jejunal MDA in the GCM group with1000 mg/kg was significantly reduced（P<0.05）.3.Effects of GCM on immune function and intestinal morphology in laying hensCompared to the control group,the addition of 250,500 and 1000 mg/kg GCM could significantly increase the serum Ig A content（P<0.05）of 24-week-old laying hens.The addition of 500 mg/kg GCM increased the serum Ig A content（P<0.05）of 24-week-old laying hens,and the addition of 250,500 and 1000 mg/kg GCM significantly increased the Ig M content in the serum of 24-week-old laying hens（P<0.05）.However,the addition of GCM group had no significant effect on the serum of Ig A,Ig G and Ig M of 28-week-old laying hens（P>0.05）.The addition of GCM significantly increased the villus height and villus height/crypt depth of the duodenum in 28-week-old laying hens（P<0.05）.Compared with the control group,the addition of 250,1000 mg/kg GCM reduced the duodenal crypt depth（P<0.05）,and the addition of 500 mg/kg GCM upregulated the villi surface area of the duodenum（P<0.05）.The addition of 250,500,and 1000 mg/kg can upregulate jejunal villus height,villus height/crypt depth（P<0.05）.Add 500 and 1000mg/kg GCM to upregulate the ileal villi height（P<0.05）;In addition,the addition of 1000mg/kg GCM increased ileal villi height/crypt depth（P<0.05）.Therefore,the addition of monoglycerides laurate and cinnamaldehyde complex to the diet can improve the egg quality,antioxidant and immune function of laying hens,and also have an effect on the intestinal morphological structure of laying hens.